Date published: 2026-5-6

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human testis extract: sc-363781

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Datasheets
  • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
  • Western blotting positive control
  • recommended use is 50 µg (20 µl) per lane
  • extracts should be stored at -20°C and repeated freezing and thawing should be minimized
  • sample vial should be placed at 95° C for up to 5 minutes, once prior to use
  • Not available in Germany.
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    Human testis extract, sourced from human testicular tissue, serves as a valuable research tool in elucidating the complex mechanisms underlying male reproductive physiology and pathology. This extract contains a diverse array of bioactive molecules, including hormones (such as testosterone), enzymes, growth factors, and structural proteins specific to the testicular microenvironment. Mechanistically, human testis extract has been extensively employed to investigate spermatogenesis, steroidogenesis, and the regulation of male reproductive function. Researchers utilize this extract to study the development and maturation of spermatozoa, as well as the modulation of testosterone synthesis and secretion by the hypothalamic-pituitary-gonadal axis. Additionally, human testis extract serves as a crucial component in cell culture models aimed at mimicking the testicular environment and exploring the effects of endogenous testicular factors on germ cell development, Leydig cell function, and intercellular communication within the testis. Studies utilizing human testis extract contribute to our understanding of male reproductive biology, providing insights into the molecular mechanisms governing fertility, spermatogenic disorders, and male infertility. Overall, human testis extract plays a pivotal role in advancing research related to male reproductive health and fertility, offering valuable avenues for the development of diagnostic assays and strategies targeting male reproductive disorders.

    human testis extract References:

    1. Specific expression of heat shock protein HspA2 in human male germ cells.  |  Son, WY., et al. 1999. Mol Hum Reprod. 5: 1122-6. PMID: 10587366
    2. Dynamic subcellular distribution of the DAZL protein is confined to primate male germ cells.  |  Ruggiu, M., et al. 2000. J Androl. 21: 470-7. PMID: 10819456
    3. Presence of antibodies to sperm YLP(12) synthetic peptide in sera and seminal plasma of immunoinfertile men.  |  Naz, RK. and Chauhan, SC. 2001. Mol Hum Reprod. 7: 21-6. PMID: 11134356
    4. Molecular cloning and sequencing of a novel cDNA encoding for a protein involved in human sperm function.  |  Naz, RK. and Zhu, X. 2001. Biochem Biophys Res Commun. 284: 911-7. PMID: 11409879
    5. Expression and function of the HSD-3.8 gene encoding a testis-specific protein.  |  Lin, W., et al. 2001. Mol Hum Reprod. 7: 811-8. PMID: 11517287
    6. Effect of antibodies to sperm-specific recombinant contraceptive vaccinogen (rCV) on murine fertilization: search for an animal model to examine its contraceptive potential.  |  Chauhan, SC. and Naz, RK. 2001. Mol Reprod Dev. 60: 425-32. PMID: 11599055
    7. Monoclonal antibody against human sperm-specific YLP12 peptide sequence involved in oocyte binding.  |  Naz, RK., et al. 2002. Arch Androl. 48: 169-75. PMID: 11964208
    8. Molecular cloning and sequencing of cDNA encoding for human FA-1 antigen.  |  Naz, RK. and Zhu, X. 2002. Mol Reprod Dev. 63: 256-68. PMID: 12203836
    9. Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm.  |  Hao, Z., et al. 2004. Mol Hum Reprod. 10: 433-44. PMID: 15044604
    10. A sperm component, HSD-3.8 (SPAG1), interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK).  |  Liu, N., et al. 2006. Front Biosci. 11: 1679-89. PMID: 16368546
    11. Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli.  |  LeVan, KM. and Goldberg, E. 1991. Biochem J. 273 (Pt 3): 587-92. PMID: 1996957
    12. Identification of the human testis protein phosphatase 1 interactome.  |  Fardilha, M., et al. 2011. Biochem Pharmacol. 82: 1403-15. PMID: 21382349
    13. Identification of a potential FSH modulatory protein in human testis and seminal plasma.  |  Sluss, PM., et al. 1989. J Androl. 10: 386-92. PMID: 2512274

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    human testis extract

    sc-363781
    500 µg/200 µl
    $120.00

    Is this testis extract from normal tissue?

    Asked by: CCCheng
    Thank you for your question. Yes, this is prepared from normal tissue. This tissue extract is recommended for use only as a Western blotting positive control for our antibodies.
    Answered by: Technical Support
    Date published: 2019-06-20
    • y_2026, m_5, d_5, h_11CST
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    Rated 1 out of 5 by from degraded proteinsI ordered this extract at the end of May 2019. It arrived frozen and I placed it immediately into -80oC freezer. a few days later, I used it for western blot. Fifty µg of cell lysate was loaded in each well of 4-20% PAGE /SDS gel and proteins were transferred to nitrocellulose membrane. The samples were: human ovary extract (sc-363769; lot# G2215), human testis extract (sc-363781; lot# I0115), as well as whole cell extracts from HEK-293 cells transiently transfected with either human ERα, or human ERβ expression vectors (made in our lab). Four identical blots were made and labelled A-D (only C and D are shown). The image below shows membranes stained with Ponceaus S, followed by overnight staining with antibodies. Blot C: anti-ERβ (Thermo, Cat# PPZ-0506; 1:1000) and anti-GAPDH (sc-32233; 1:5000) Blot D: anti-ERα (sc-8002; 1:2000) and anti-GAPDH (sc-32233; 1:5000). Clearly, both ovary and testis extracts were of poor quality: did not show expression of ERα, or ERβ although those tissues are known to express both proteins. Moreover, even GAPDH staining didn’t show any bands. I sent a request to SCBT for a datasheet. but got a reply saying: "We do not provide a datasheet for human testis extract: sc-363781 For more information on this product, please visit our website: https://www.scbt.com/scbt/search?Ntt=sc-363781". The problem is that there is NO more information on that site. Between 6/10/2019 and 6/17/2019 I sent 4 (FOUR!) emails complaining about the poor quality of the extracts and requesting new AND TESTED batches of them, but I have never received any response. Very disappointing!
    Date published: 2019-06-27
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    human testis extract is rated 1.0 out of 5 by 1.
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