Date published: 2026-5-6

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human liver extract: sc-363766

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Datasheets
  • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
  • Western blotting positive control
  • recommended use is 50 µg (20 µl) per lane
  • extracts should be stored at -20°C and repeated freezing and thawing should be minimized
  • sample vial should be placed at 95° C for up to 5 minutes, once prior to use
  • Not available in Germany.
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Human liver extract, originating from human liver tissue, serves as a fundamental tool in biomedical research, particularly in studies related to hepatic physiology, metabolism, and disease. The liver plays a central role in various physiological processes, including metabolism, detoxification, and immune modulation. Human liver extracts contain a diverse array of proteins, enzymes, and metabolites that reflect the complex functions of the liver microenvironment. Researchers utilize these extracts to investigate the molecular mechanisms underlying liver diseases such as hepatitis, cirrhosis, and liver cancer. By analyzing the composition and activity of specific enzymes and proteins present in the liver tissue, researchers can identify biomarkers for disease diagnosis, progression, and prognosis. Additionally, human liver extracts are invaluable in studying drug metabolism and toxicity, as the liver is the primary organ responsible for drug metabolism and detoxification. Furthermore, these extracts are utilized in the development of in vitro models of liver tissue to study liver biology and disease pathogenesis, offering insights into potential interventions for liver disorders. Overall, human liver extracts play a crucial role in advancing our understanding of liver physiology and pathology, contributing to the development of novel diagnostic approaches for liver diseases.

human liver extract References:

  1. Inhibition of protein and nucleic acid synthesis of animal cells in vitro mediated by high molecular weight inhibitors in human liver extract.  |  Nilsson, G. 1976. Biochim Biophys Acta. 418: 376-96. PMID: 1247551
  2. Canaline carbamoyltransferase in human liver as part of a metabolic cycle in which guanidino compounds are formed.  |  Natelson, S., et al. 1977. Clin Chem. 23: 960-6. PMID: 15744
  3. Liver Galbeta1,4GlcNAc alpha2,6-sialyltransferase is down-regulated in human alcoholics: possible cause for the appearance of asialoconjugates.  |  Gong, M., et al. 2007. Metabolism. 56: 1241-7. PMID: 17697868
  4. Purification of human liver guanase and characterization of antibody against it by immunoblotting.  |  Ito, S., et al. 1990. Clin Biochem. 23: 113-20. PMID: 2372926
  5. Characterization of antibody against human liver guanase by immunoblotting and immunohistochemical staining of human liver guanase by a direct labeling antibody-enzyme method.  |  Ito, S., et al. 1989. J Histochem Cytochem. 37: 611-5. PMID: 2649557
  6. Preparation and properties of a specific antiserum against human liver.  |  Mihas, AA. and Spenney, JG. 1977. Int Arch Allergy Appl Immunol. 54: 255-61. PMID: 406206
  7. Hydrolysis of Tay-Sachs ganglioside by beta-hexosaminidase A of human liver and urine.  |  Li, YT., et al. 1973. J Biol Chem. 248: 7512-5. PMID: 4745777
  8. Phenylalanine hydroxylase of human liver: assay and some properties.  |  Kaufman, S. 1969. Arch Biochem Biophys. 134: 249-52. PMID: 5345589
  9. Thymidine and uridine metabolism at cell growth inhibition of HeLa cells by human liver extract.  |  Nilsson, G. 1970. Exp Cell Res. 59: 207-16. PMID: 5413543
  10. Oxidation of 7-methylguanine by human xanthine oxidase.  |  Skupp, S. and Ayvazian, JH. 1969. J Lab Clin Med. 73: 909-16. PMID: 5815015
  11. Removal of O6-methylguanine from DNA by human liver fractions.  |  Pegg, AE., et al. 1982. Proc Natl Acad Sci U S A. 79: 5162-5. PMID: 6957855
  12. Regulation of human lymphocyte function by a soluble extract from normal human liver.  |  Chisari, FV. 1978. J Immunol. 121: 1279-86. PMID: 701796
  13. 2-Aminoadipate-2-oxoglutarate aminotransferase isoenzymes in human liver: a plausible physiological role in lysine and tryptophan metabolism.  |  Okuno, E., et al. 1993. Enzyme Protein. 47: 136-48. PMID: 8087205

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

human liver extract

sc-363766
500 µg/200 µl
$120.00

If I want to dilute this further, in example loading 5ug, which would be adding 2uL per lane. Do you recommend a buffer that I can dilute it in?

Asked by: Anonymous
Thank you for your question. This extract is provided in SDS-PAGE Western blotting buffer, which would be appropriate for diluting it to the desired concentration.
Answered by: Tech Support Europe
Date published: 2022-04-28

Can you use and how much of the extract to dissolve and with what?

Asked by: Vygantas
Thank you for your question. This product, human liver extract: sc-363766, is provided ready to use as 500 µg protein in 200 µl SDS-PAGE Western blotting buffer. We recommend loading 50 µg (20 µl) per lane for use as a positive control in Western blotting. There is no need to dissolve the extract in any buffer. The sample vial should be boiled once prior to use.
Answered by: Technical Support
Date published: 2018-03-29
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Rated 5 out of 5 by from marioperfect for consumption ligma balls sugondees nuts
Date published: 2023-03-01
Rated 5 out of 5 by from We used this control in WB and worked wellWe used this control in WB and worked well.
Date published: 2015-08-31
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human liver extract is rated 5.0 out of 5 by 2.
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