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Santa Cruz Biotechnology Human Insulin, Solution (sc-360248), 5 ml vial, ideal for research applications requiring human i
Santa Cruz Biotechnology Human Insulin, Solution (sc-360248), 5 ml vial, ideal for research applications requiring human insulin.
Santa Cruz Biotechnology Human Insulin, Solution (sc-360248), 5 ml vial, ideal for research applications requiring human i
Santa Cruz Biotechnology Human Insulin, Solution (sc-360248), 5 ml vial, ideal for research applications requiring human insulin.
Human Insulin, Solution, product number sc-360248 by Santa Cruz Biotechnology, high-purity insulin for reliable research r
Human Insulin, Solution, product number sc-360248 by Santa Cruz Biotechnology, high-purity insulin for reliable research results.
Santa Cruz Biotechnology Human Insulin, Solution (sc-360248), 5 ml vial, ideal for research applications requiring human insulin.

Human Insulin, Solution (CAS 11061-68-0)

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Alternate Names:
Human Insulin, Solution also known as Insulin; human insulin
Application:
Human Insulin, Solution is derived from Escherichia Coli., 10mg/ml, 0.2µm filtered, for use in cell culture applications
CAS Number:
11061-68-0
Molecular Weight:
5807.57
Molecular Formula:
C257H383N65O77S6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Technical Information
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SDS & Certificate of Analysis

Human Insulin, Solution is a protein preparation used extensively in biochemical and physiological research. It is employed in studies exploring insulin signaling pathways and glucose metabolism, providing insights into the regulation of blood sugar levels and energy homeostasis. Researchers use Human Insulin, Solution to stimulate insulin receptors in cell cultures and to investigate insulin′s role in cellular processes such as glycogen synthesis, lipid metabolism, and protein synthesis. In diabetes research, this solution is applied to understand the mechanisms of insulin resistance and to explore the function of pancreatic beta cells. Additionally, Human Insulin, Solution is used in assays to assess the insulin sensitivity of various tissues and to study the downstream effects of insulin binding on target cells.


Human Insulin, Solution (CAS 11061-68-0) References

  1. Development of two high-performance liquid chromatographic methods for the analysis and characterization of insulin and its degradation products in pharmaceutical preparations.  |  Oliva, A., et al. 2000. J Chromatogr B Biomed Sci Appl. 749: 25-34. PMID: 11129075
  2. Transdermal delivery of insulin from a novel biphasic lipid system in diabetic rats.  |  King, MJ., et al. 2002. Diabetes Technol Ther. 4: 479-88. PMID: 12396742
  3. Improved sensitivity for insulin in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by premixing alpha-cyano-4-hydroxycinnamic acid matrix with transferrin.  |  Kobayashi, T., et al. 2004. Rapid Commun Mass Spectrom. 18: 1156-60. PMID: 15150841
  4. Role of insulin signaling in maintaining energy homeostasis.  |  Sharma, MD., et al. 2008. Endocr Pract. 14: 373-80. PMID: 18463047
  5. Evaluation of hydrophobic nanoparticulate delivery system for insulin.  |  Singnurkar, PS. and Gidwani, SK. 2008. Indian J Pharm Sci. 70: 721-6. PMID: 21369431
  6. Insulin concentration is critical in culturing human neural stem cells and neurons.  |  Rhee, YH., et al. 2013. Cell Death Dis. 4: e766. PMID: 23928705
  7. Sensitive detection of human insulin using a designed combined pore approach.  |  Lei, C., et al. 2014. Small. 10: 2413-8. PMID: 24599559
  8. Combined effects of potassium chloride and ethanol as mobile phase modulators on hydrophobic interaction and reversed-phase chromatography of three insulin variants.  |  Johansson, K., et al. 2015. J Chromatogr A. 1381: 64-73. PMID: 25595534
  9. Osteoinductive activity of insulin-functionalized cell culture surfaces obtained using diazonium chemistry.  |  Mikulska, A., et al. 2014. Front Chem. 2: 117. PMID: 25629028
  10. Glucose and Insulin Tolerance Tests in the Mouse.  |  Vinué, Á. and González-Navarro, H. 2015. Methods Mol Biol. 1339: 247-54. PMID: 26445794
  11. Synthesis and Identification of FITC-Insulin Conjugates Produced Using Human Insulin and Insulin Analogues for Biomedical Applications.  |  Jacob, D., et al. 2016. J Fluoresc. 26: 617-29. PMID: 26658795
  12. Surface-Enhanced Raman Spectroscopy-Based Label-Free Insulin Detection at Physiological Concentrations for Analysis of Islet Performance.  |  Cho, H., et al. 2018. ACS Sens. 3: 65-71. PMID: 29322773
  13. Characterisation of nasal devices for delivery of insulin to the brain and evaluation in humans using functional magnetic resonance imaging.  |  Wingrove, J., et al. 2019. J Control Release. 302: 140-147. PMID: 30953665
  14. Zinc determines dynamical properties and aggregation kinetics of human insulin.  |  Pounot, K., et al. 2021. Biophys J. 120: 886-898. PMID: 33545104
  15. A case of primary and generalized allergy to human insulin with no history of any prior insulin exposure.  |  Takuma, H., et al. 1995. Diabetes Res Clin Pract. 30: 69-73. PMID: 8745208

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Human Insulin, Solution, 5 ml

sc-360248
5 ml
$176.00

What is the shelf life of the product when stored at 4 degrees? Can it be frozen -20? Thanks

Asked by: Hagai
Thank you for your question. sc-360248 Human Insulin, Solution has a retest of 5 years. The recommended storage is 4 degrees.
Answered by: BlakeJ
Date published: 2024-09-20

Hi, How it is possible to prepare a solution containing 200 nM insulin from Human Insulin, Solution (CAS 11061-68-0)?? and is this reagent suitable for a study on RBL-2H3 cell culture for a study related to diabetes or Insulin: sc-29062 reagent ??

Asked by: Anonymous
Thank you for your question. We have not tested this in a cell culture application so we can not make any recommendation. sc-360248 contains 9.5-10.5 mg/ml recombinant Human insulin in 25mM HEPES buffer, pH: 7.9 - 8.5.
Answered by: Tech Service
Date published: 2019-12-02

What is the pH of this solution? Is insulin readily soluble in 25mM HEPES buffer?

Asked by: mkharini
pH: 7.9 - 8.5 sc-360248 Contains 9.5-10.5 mg/ml recombinant Human insulin in 25mM HEPES buffer.
Answered by: Technical Support
Date published: 2019-04-22

Is it suitable for in vivo models in mices?

Asked by: Confused student
Thank you for your question. We do not recommend using our Human Insulin, Solution: sc-360248, for in vivo research applications. Our compounds are not pharmaceutical grade and not manufactured under GMP guidelines. Please consult your principal investigator and your animal care and use committee (ACUC) regarding the use of our compounds in animals. We recommend this for research use only in cell culture applications.
Answered by: Technical Support
Date published: 2017-08-23

Hello, I was wondering how ling is this insulin solution stable after opening and constantly taking aliquots once in a while assuming it is being kept at 4° C?

Asked by: BioMate68
Thank you for your question. Product is stable and suitable for use for 12 months following purchase (stored properly, unopened). The solution can be aliquoted and stored at -20°C. Multiple freeze-thaw cycles should be avoided. Product is supplied sterile, recommend aliquots should be prepared using proper aseptic techniques to insure product is not contaminated. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2023-03-22

Can this be used for human cell culture? for therapeutic applications?

Asked by: mukesh
Thank you for your question. We offer this reagent, Human Insulin, Solution: sc-360248, for research use only. It is not intended for diagnostic or therapeutic use. This solution is suitable for use in mammalian cell culture applications at 0.5 mL to 1 mL per liter of medium.
Answered by: Technical Support
Date published: 2017-05-12

Hi what is the maximum shelf-life of santa cruz insulin solution at 4C?

Asked by: Farjam
Thank you for your question. This Insulin product is stable and suitable for use for 12 months following purchase (stored properly, unopened).
Answered by: Tech Service
Date published: 2023-07-15

What is the molarity?

Asked by: Jz28sail
Thank you for your question. sc-360248 contains 9.5-10.5 mg/ml recombinant Human insulin in 25mM HEPES buffer. The molarity can be calculated. The molecular weight of recombinant human insulin is approx. 5800. Also available: sc-29062, Recombinant Human Insulin: Biological Activity: 28 units/mg.
Answered by: Technical Support
Date published: 2018-06-11
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Rated 5 out of 5 by from Human insulin solutionReady to use formulation is easy to use. My cells are growing well.
Date published: 2022-09-12
Rated 5 out of 5 by from Happy 10as, happy customerWe bought this to replace the sigma insulin which has been out of stock all year. Our MCF10as don't seem to have noticed the difference.
Date published: 2022-08-17
Rated 5 out of 5 by from This Works Well!It works well. Used this for culturing organoids. Needed in deveolping media!
Date published: 2022-07-05
Rated 5 out of 5 by from Human Isulin: Very good experience.We purchased recently Human insulin and found that it was very good and cheap compare to other brands. We got very good results. Thank you !! Sonu
Date published: 2021-09-08
Rated 5 out of 5 by from Great for researchthis is a vital reagent for our lab's experiments!
Date published: 2021-08-30
Rated 5 out of 5 by from No complaintsNo complaints. Used in cell culture.
Date published: 2015-09-04
Rated 5 out of 5 by from Recombinant human insulin is used in researchRecombinant human insulin is used in research as a supplement in cell culture media to support various mammalian cell lines that require this additional hormone to uptake glucose and amino acids. -SCBT Publication Review
Date published: 2015-01-28
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Human Insulin, Solution is rated 5.0 out of 5 by 7.
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