human adipose tissue extract: sc-363750
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- Western blotting positive control
- recommended use is 50 µg (20 µl) per lane
- extracts should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
- Not available in Germany.
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Human adipose tissue extract, a derivative concentrated from the adipose tissue commonly found in the human body, primarily consists of various cellular components and soluble proteins. This extract is obtained through a meticulous process where adipose cells are harvested and then subjected to lysis, releasing their intracellular contents, which include a rich array of cytokines, growth factors, and extracellular matrix proteins. Historically, the value of this extract in research stems from its complex biological composition, which mirrors many of the physiological conditions present within the human body. Scientists utilize this extract extensively in the field of bioengineering and regenerative research, particularly in studies aimed at understanding adipogenesis and the signaling pathways involved in fat metabolism and storage. Moreover, its application in the development of 3D tissue models offers profound insights into cellular behaviors and tissue responses in a controlled environment, facilitating a deeper understanding of cellular dynamics without the influence of systemic factors. The extract's rich content also supports studies in cellular nutrition and energy balance, providing a near-physiological context that helps delineate the mechanisms by which cells process and respond to nutritional cues. Thus, human adipose tissue extract serves as a pivotal tool in advancing our knowledge of cellular and molecular biology, contributing significantly to foundational scientific inquiries.
human adipose tissue extract References:
- Determination of polybrominated diphenyl ethers and polychlorinated biphenyls in human adipose tissue by large-volume injection-narrow-bore capillary gas chromatography/electron impact low-resolution mass spectrometry. | Covaci, A., et al. 2002. Anal Chem. 74: 790-8. PMID: 11866059
- On the individuality of aliphatic and alicyclic monoester lipases in human adipose tissue. | Charbonnier, M. and Boyer, J. 1976. Biochim Biophys Acta. 424: 329-36. PMID: 1259963
- Determination of corticosteroids in tissue samples by liquid chromatography-tandem mass spectrometry. | Rönquist-Nii, Y. and Edlund, PO. 2005. J Pharm Biomed Anal. 37: 341-50. PMID: 15708676
- Human adipose tissue extract induces angiogenesis and adipogenesis in vitro. | Sarkanen, JR., et al. 2012. Tissue Eng Part A. 18: 17-25. PMID: 21902602
- Optimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatography. | Fernandes, VC., et al. 2012. Biomed Chromatogr. 26: 1494-501. PMID: 22407478
- Adipose tissue extract promotes adipose tissue regeneration in an adipose tissue engineering chamber model. | Lu, Z., et al. 2016. Cell Tissue Res. 364: 289-98. PMID: 26678825
- Cytokine-Rich Adipose Tissue Extract Production from Water-Assisted Lipoaspirate: Methodology for Clinical Use. | Lopez, J., et al. 2016. Biores Open Access. 5: 269-78. PMID: 27679753
- Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying. | Auvinen, VV., et al. 2019. Cryobiology. 91: 137-145. PMID: 31533026
- Optimization of a simple, effective, and greener methodology for polycyclic aromatic hydrocarbon extraction from human adipose tissue. | Sousa, S., et al. 2023. Anal Methods. 15: 1722-1733. PMID: 36938680
- Ethyl and methyl oleate: a pitfall in the assay of adipose tissue glycerol ester hydrolase activity. | Boyer, J., et al. 1971. Biochim Biophys Acta. 239: 353-6. PMID: 4330335
- Affinity chromatography of human adipose tissue triacylglycerol lipase on agarose-linked dioleoylglycerol. | Verine, A., et al. 1974. Biochim Biophys Acta. 369: 125-8. PMID: 4422493
- Identification of polybrominated biphenyls in the adipose tissues of the general population of the United States. | Lewis, RG. and Sovocool, GW. 1982. J Anal Toxicol. 6: 196-8. PMID: 6289008
- Evaluation of gel permeation chromatography for clean up of human adipose tissue samples for GC/MS analysis of pesticides and other chemicals. | MacLeod, KE., et al. 1982. J Anal Toxicol. 6: 38-40. PMID: 7078104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
human adipose tissue extract | sc-363750 | 500 µg/200 µl | $120.00 |