HSV-1 UL42 Antibody (13C9): sc-53331
- HSV-1 UL42 Antibody (13C9) is a mouse monoclonal IgG1 κ HSV-1 UL42 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against Baculovirus-expressed HSV DNA polymerase (POL) and POL/UL42 complex
- HSV-1 UL42 Antibody (13C9) is recommended for detection of UL42 of HSV-1 origin by WB, IP and IF
- Anti-HSV-1 UL42 Antibody (13C9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HSV-1 UL42 Antibody (13C9). This work is in progress.
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HSV-1 UL42 Antibody (13C9) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HSV-1 UL42 protein of HSV-1 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). HSV-1 UL42 Antibody (13C9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The herpes simplex virus (HSV), commonly known for causing cold sores, is a highly contagious virus that can establish latent infections in the host, leading to potential reactivation and disease. UL42 plays a critical role in the viral life cycle as a processivity factor for the HSV-1 DNA polymerase, which is essential for viral DNA replication. UL42 enhances the stability of the DNA polymerase on the primer-template DNA, thereby increasing the efficiency of viral genome replication. UL42′s ability to facilitate the loading of UL9 onto DNA is crucial, as UL42 forms a functional complex that unwinds duplex DNA, a necessary step for replication. Understanding UL42′s function is vital for developing antiviral strategies, as targeting UL42 could disrupt the replication process of the virus, offering potential therapeutic avenues for managing HSV infections.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HSV-1 UL42 Antibody (13C9) References:
- The crystal structure of an unusual processivity factor, herpes simplex virus UL42, bound to the C terminus of its cognate polymerase. | Zuccola, HJ., et al. 2000. Mol Cell. 5: 267-78. PMID: 10882068
- Analysis of in vitro activities of herpes simplex virus type 1 UL42 mutant proteins: correlation with in vivo function. | Thornton, KE., et al. 2000. Virology. 275: 373-90. PMID: 10998337
- Evidence against a simple tethering model for enhancement of herpes simplex virus DNA polymerase processivity by accessory protein UL42. | Chaudhuri, M. and Parris, DS. 2002. J Virol. 76: 10270-81. PMID: 12239303
- The herpes simplex virus processivity factor, UL42, binds DNA as a monomer. | Randell, JC. and Coen, DM. 2004. J Mol Biol. 335: 409-13. PMID: 14672651
- The herpes simplex virus type 1 DNA polymerase processivity factor, UL42, does not alter the catalytic activity of the UL9 origin-binding protein but facilitates its loading onto DNA. | Trego, KS., et al. 2005. Nucleic Acids Res. 33: 536-45. PMID: 15673714
- Effects of substitutions of arginine residues on the basic surface of herpes simplex virus UL42 support a role for DNA binding in processive DNA synthesis. | Randell, JC., et al. 2005. J Virol. 79: 12025-34. PMID: 16140778
- The essential in vivo function of the herpes simplex virus UL42 protein correlates with its ability to stimulate the viral DNA polymerase in vitro. | Reddig, PJ., et al. 1994. Virology. 200: 447-56. PMID: 8178434
- Characterization of monoclonal antibodies recognizing amino- and carboxy-terminal epitopes of the herpes simplex virus UL42 protein. | Sheaffer, AK., et al. 1995. Virus Res. 38: 305-14. PMID: 8578868
- Herpes simplex virus processivity factor UL42 imparts increased DNA-binding specificity to the viral DNA polymerase and decreased dissociation from primer-template without reducing the elongation rate. | Weisshart, K., et al. 1999. J Virol. 73: 55-66. PMID: 9847307
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSV-1 UL42 Antibody (13C9) | sc-53331 | 200 µg/ml | $322.00 | |||
HSV-1 UL42 Antibody (13C9) AC | sc-53331 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HSV-1 UL42 Antibody (13C9) HRP | sc-53331 HRP | 200 µg/ml | $322.00 | |||
HSV-1 UL42 Antibody (13C9) FITC | sc-53331 FITC | 200 µg/ml | $336.00 | |||
HSV-1 UL42 Antibody (13C9) PE | sc-53331 PE | 200 µg/ml | $349.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 488 | sc-53331 AF488 | 200 µg/ml | $364.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 546 | sc-53331 AF546 | 200 µg/ml | $364.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 594 | sc-53331 AF594 | 200 µg/ml | $364.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 647 | sc-53331 AF647 | 200 µg/ml | $364.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 680 | sc-53331 AF680 | 200 µg/ml | $364.00 | |||
HSV-1 UL42 Antibody (13C9) Alexa Fluor® 790 | sc-53331 AF790 | 200 µg/ml | $364.00 |