HSPA1L Double Nickase Plasmid (h): sc-400479-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA1L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSPA1L Double Nickase Plasmid (h) and HSPA1L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPA1L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA1L Antibody (C-6): sc-393297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA1L Double Nickase Plasmid (h) | sc-400479-NIC | 20 µg | $410.00 | |||
HSPA1L Double Nickase Plasmid (h2) | sc-400479-NIC-2 | 20 µg | $410.00 |
HSPA1L encodes a stress-inducible member of the HSP70 chaperone family that supports protein homeostasis by binding unfolded polypeptides and coordinating their refolding, trafficking, or triage for degradation. HSPA1L participates in cellular proteostasis networks including heat-shock response signaling, co-chaperone–regulated ATPase cycles, and crosstalk with ubiquitin–proteasome and autophagy pathways during proteotoxic stress. By modulating protein folding capacity and stress resilience, HSPA1L is relevant to contexts where chaperone balance influences cell survival, differentiation, and immune or reproductive biology. Dysregulated chaperone activity has been associated with altered stress tolerance and disease-linked protein quality control, making HSPA1L a useful target for mechanistic studies of proteostasis.
HSPA1L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPA1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPA1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPA1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPA1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.