HSP70 (h): 293T Lysate: sc-116686

HSP 70 (h): 293T Lysate is derived from the HEK 293T cell line, specifically engineered to facilitate the study of the Heat Shock Protein 70 (HSP70) family's role in cellular processes. This lysate is invaluable for research focused on the molecular mechanisms of protein folding, stabilization, and repair processes under stress conditions. HEK 293T cells, a human embryonic kidney cell line, have been modified to express the SV40 large T antigen, which enhances the propagation of plasmids containing the SV40 origin of replication. This feature makes the lysate ideal for high-efficiency transfection applications, enabling the detailed study of gene expression and protein interactions. Researchers utilize HSP 70 (h): 293T Lysate to investigate HSP70's interaction with nascent polypeptides and its role in preventing their aggregation, thereby facilitating proper protein folding. This research has profound implications for understanding the cellular mechanisms that maintain proteostasis and cellular homeostasis in non-clinical contexts. Through techniques such as co-immunoprecipitation, Western blotting, and protein assays, scientists explore how HSP70 mediates the cellular response to physiological stress, crucial for uncovering fundamental cellular mechanisms.