Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSL Double Nickase Plasmid (h): sc-401341-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSL Double Nickase Plasmid (h) and HSL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LIPE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSL Antibody (G-7): sc-74489
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSL Double Nickase Plasmid (h) | sc-401341-NIC | 20 µg | $410.00 | |||
HSL Double Nickase Plasmid (h2) | sc-401341-NIC-2 | 20 µg | $410.00 |
Human LIPE encodes hormone-sensitive lipase (HSL), a serine hydrolase that catalyzes key steps in neutral lipid mobilization by hydrolyzing diacylglycerols and other lipid esters in adipocytes and steroidogenic tissues. HSL activity is dynamically controlled by PKA- and AMPK-linked phosphorylation and integrates adrenergic and insulin signaling to regulate lipolysis, lipid droplet remodeling, and cellular energy balance. Through its role in fatty acid flux and lipid signaling intermediates, LIPE influences metabolic pathway wiring, including glycerolipid metabolism and downstream mitochondrial substrate utilization. Altered HSL function and LIPE regulation are studied in the context of metabolic dysregulation, adipose tissue inflammation, and insulin resistance–associated phenotypes.
HSL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LIPE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LIPE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LIPE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LIPE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.