HSFY1 Double Nickase Plasmid (h): sc-417414-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSFY1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSFY1 Double Nickase Plasmid (h) and HSFY1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSFY1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSFY1 Double Nickase Plasmid (h) | sc-417414-NIC | 20 µg | $410.00 | |||
HSFY1 Double Nickase Plasmid (h2) | sc-417414-NIC-2 | 20 µg | $410.00 |
HSFY1 (heat shock transcription factor, Y-linked 1) encodes a Y chromosome–associated member of the heat shock factor family implicated in transcriptional regulation programs that support cellular proteostasis. Although less characterized than canonical HSFs, HSFY1 is linked to stress-responsive gene expression, protein folding homeostasis, and quality-control pathways that coordinate chaperone networks and adaptation to proteotoxic conditions. Expression and genetic variation of HSFY1 have been studied in the context of male germline biology, with reported associations to impaired spermatogenesis and Y chromosome–linked infertility phenotypes. As a transcriptional regulator, HSFY1 provides a tractable entry point for dissecting how sex-linked regulatory factors modulate stress signaling and reproductive cell state.
HSFY1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSFY1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSFY1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSFY1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSFY1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.