Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSF1 CRISPR/Cas9 KO Plasmid (h2): sc-400432-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSF1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSF1 Antibody (E-4): sc-17757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSF1 CRISPR/Cas9 KO Plasmid (h2) | sc-400432-KO-2 | 20 µg | $397.00 |
Overview
Heat shock factor 1 (HSF1) is a master transcriptional regulator that coordinates the cellular heat shock response by inducing chaperones and proteostasis genes, including HSP70 and HSP90 family members, to preserve protein folding and limit proteotoxic stress. Beyond acute stress adaptation, HSF1 integrates signals from proteasome function, translation, and metabolic state to influence protein quality control, cytoskeletal organization, and cell survival programs. HSF1 activity intersects with pathways such as HSP90-dependent client stabilization, autophagy, and stress granule dynamics, shaping how cells respond to environmental and intracellular stressors. Dysregulated HSF1 signaling has been implicated in malignant transformation and tumor maintenance, as well as neurodegeneration and other protein-misfolding–associated disorders, making it a widely used node for mechanistic studies.
HSF1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the HSF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HSF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HSF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSF1 protein expression.
This CRISPR knockout system enables efficient generation of HSF1-deficient cell models for investigation of HSF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HSF1 exon(s) critical for HSF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HSF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSF1 CRISPR/Cas9 KO Plasmid (h) and HSF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HSF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSF1 HDR Plasmid (h) and HSF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HSF1 homology arms to support homology-directed repair at defined HSF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.