HRT1 Antibody (NB-A7): sc-134362

HRT1 Antibody (NB-A7) is a mouse monoclonal IgG2a kappa light chain antibody that detects HRT1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-HRT1 antibody (NB-A7) is available as a non-conjugated format. HRT1, a member of the Hairy/Enhancer of split (HES) family, plays a crucial role in regulating gene expression and cell fate determination during embryonic development. HRT1 is particularly important in Notch signaling, which activates HRT1 and other HES genes, leading to basic helix-loop-helix (bHLH) transcriptional repressors essential for proper embryonic patterning. HRT1 is predominantly located in somitic mesoderm, central nervous system, kidney, heart, nasal epithelium, and limb buds during murine embryogenesis, with expression also observed in various adult tissues. HRT1 has been implicated in altered expression patterns in several cancers, including breast, lung, and kidney tumors, highlighting potential as a biomarker for tumorigenesis. Structural features of HRT1, including bHLH domain, Orange domain, and unique YRPW domain, contribute to functional specificity and interaction with other proteins in Notch signaling pathway, making HRT1 a vital component in developmental biology and cancer research.

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HRT1 Antibody (NB-A7) References:

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2. Identification and expression of a novel family of bHLH cDNAs related to Drosophila hairy and enhancer of split.  |  Kokubo, H., et al. 1999. Biochem Biophys Res Commun. 260: 459-65. PMID: 10403790
3. Hey genes: a novel subfamily of hairy- and Enhancer of split related genes specifically expressed during mouse embryogenesis.  |  Leimeister, C., et al. 1999. Mech Dev. 85: 173-7. PMID: 10415358
4. HRT1, HRT2, and HRT3: a new subclass of bHLH transcription factors marking specific cardiac, somitic, and pharyngeal arch segments.  |  Nakagawa, O., et al. 1999. Dev Biol. 216: 72-84. PMID: 10588864
5. Characterization of the human and mouse HEY1, HEY2, and HEYL genes: cloning, mapping, and mutation screening of a new bHLH gene family.  |  Steidl, C., et al. 2000. Genomics. 66: 195-203. PMID: 10860664
6. Analysis of HeyL expression in wild-type and Notch pathway mutant mouse embryos.  |  Leimeister, C., et al. 2000. Mech Dev. 98: 175-8. PMID: 11044625
7. The basic helix-loop-helix transcription factor HESR1 regulates endothelial cell tube formation.  |  Henderson, AM., et al. 2001. J Biol Chem. 276: 6169-76. PMID: 11069914
8. Members of the HRT family of basic helix-loop-helix proteins act as transcriptional repressors downstream of Notch signaling.  |  Nakagawa, O., et al. 2000. Proc Natl Acad Sci U S A. 97: 13655-60. PMID: 11095750
9. HRT1 modulates vascular smooth muscle cell proliferation and apoptosis.  |  Wang, W., et al. 2003. Biochem Biophys Res Commun. 308: 596-601. PMID: 12914792
10. Pharyngeal arch artery defects and lethal malformations of the aortic arch and its branches in mice deficient for the Hrt1/Hey1 transcription factor.  |  Fujita, M., et al. 2016. Mech Dev. 139: 65-73. PMID: 26577899
11. A phase 1/2, open-label, parallel group study to evaluate the preliminary efficacy and usability DARE-HRT1 (80 μg estradiol/4 mg progesterone and 160 μg estradiol/8 mg progesterone intravaginal RinGSM) over 12 weeks in healthy postmenopausal women.  |  Thurman, A., et al. 2023. Menopause. 30: 940-946. PMID: 37625088