Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HPS-1 Double Nickase Plasmid (h): sc-404200-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HPS-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HPS-1 Double Nickase Plasmid (h) and HPS-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HPS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HPS-1 Antibody (5G12G2): sc-101435
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPS-1 Double Nickase Plasmid (h) | sc-404200-NIC | 20 µg | $410.00 | |||
HPS-1 Double Nickase Plasmid (h2) | sc-404200-NIC-2 | 20 µg | $410.00 |
HPS1 encodes HPS-1, a core component of the biogenesis of lysosome-related organelles complex-3 (BLOC-3) that regulates endosomal trafficking and maturation of lysosome-related organelles. Through its partnership with HPS4, HPS-1 supports Rab GTPase-dependent membrane transport processes that influence cargo sorting, organelle identity, and vesicle dynamics in pigment cells, platelets, and immune lineages. Disruption of HPS1 perturbs lysosomal and melanosomal pathways, affecting organelle biogenesis and intracellular protein trafficking. Variants in HPS1 are associated with Hermansky–Pudlak syndrome, linking BLOC-3 dysfunction to defects in pigmentation, platelet dense granule formation, and broader lysosome-related organelle homeostasis.
HPS-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HPS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HPS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HPS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HPS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.