HPRT Antibody (F-1): sc-376938

HPRT Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPRT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-HPRT antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPRT, also known as hypoxanthine phosphoribosyltransferase 1, is a crucial 218 amino acid cytoplasmic protein that plays a significant role in purine metabolism as a purine salvage enzyme. HPRT catalyzes the conversion of hypoxanthine and guanine into their respective mononucleotides, inosine monophosphate and guanosine monophosphate, thereby facilitating purine recycling and maintaining nucleotide balance within cells. HPRT exists as a homotetramer and requires two magnesium ions as cofactors for enzymatic activity. Deficiencies in HPRT can lead to serious metabolic disorders such as gout and Lesch-Nyhan syndrome, characterized by a lack of enzymatic activity. HPRT′s role in purine metabolism is underscored by severe consequences of dysfunction, including hyperuricemia and neurological symptoms associated with Lesch-Nyhan syndrome, highlighting this enzyme′s critical nature in cellular health and metabolic homeostasis.

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HPRT Antibody (F-1) References:

1. A recurrent large Alu-mediated deletion in the hypoxanthine phosphoribosyltransferase (HPRT1) gene associated with Lesch-Nyhan syndrome.  |  Mizunuma, M., et al. 2001. Hum Mutat. 18: 435-43. PMID: 11668636
2. An inactive X specific replication origin associated with a matrix attachment region in the human X linked HPRT gene.  |  Koina, E. and Piper, A. 2005. J Cell Biochem. 95: 391-402. PMID: 15779006
3. Normal HPRT coding region in a male with gout due to HPRT deficiency.  |  Dawson, PA., et al. 2005. Mol Genet Metab. 85: 78-80. PMID: 15862284
4. HPRTSardinia: a new point mutation causing HPRT deficiency without Lesch-Nyhan disease.  |  Cossu, A., et al. 2006. Biochim Biophys Acta. 1762: 29-33. PMID: 16216473
5. Gene duplication and inactivation in the HPRT gene family.  |  Keebaugh, AC., et al. 2007. Genomics. 89: 134-42. PMID: 16928426
6. HPRT mutations in lymphocytes from 1,3-butadiene-exposed workers in China.  |  Liu, S., et al. 2008. Environ Health Perspect. 116: 203-8. PMID: 18288319
7. A review of HPRT and its emerging role in cancer.  |  Townsend, MH., et al. 2018. Med Oncol. 35: 89. PMID: 29730818
8. HPRT-related hyperuricemia with a novel p.V35M mutation in HPRT1 presenting familial juvenile gout.  |  Mishima, E., et al. 2020. CEN Case Rep. 9: 210-214. PMID: 32128695
9. HPRT: gene structure, expression, and mutation.  |  Stout, JT. and Caskey, CT. 1985. Annu Rev Genet. 19: 127-48. PMID: 3909940
10. Analysis of mutation at the hprt locus in human T lymphocytes.  |  Lambert, B., et al. 1995. Toxicol Lett. 82-83: 323-33. PMID: 8597072
11. An asymptomatic germline missense base substitution in the hypoxanthine phosphoribosyltransferase (HPRT) gene that reduces the amount of enzyme in humans.  |  Fujimori, S., et al. 1997. Hum Genet. 99: 8-10. PMID: 9003484

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPRT Antibody (F-1) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-HPRT Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPRT Antibody (F-1) Specifications

Alternate Product Name: HPRT1

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 167-201

Epitope Target: HPRT

Epitope Species: human

Product Reactivity/Detection: HPRT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for HPRT Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin