Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HP1γ Double Nickase Plasmid (h): sc-417205-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HP1γ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HP1γ Double Nickase Plasmid (h) and HP1γ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CBX3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HP1γ Antibody (F-1): sc-398562
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HP1γ Double Nickase Plasmid (h) | sc-417205-NIC | 20 µg | $410.00 | |||
HP1γ Double Nickase Plasmid (h2) | sc-417205-NIC-2 | 20 µg | $410.00 |
CBX3 encodes heterochromatin protein 1 gamma (HP1γ), a chromatin reader that binds methylated histone H3 lysine 9 (H3K9me) via its chromodomain and contributes to heterochromatin organization. HP1γ participates in epigenetic regulation of transcription, maintenance of genome stability, and coordination of DNA replication and repair through dynamic chromatin compaction. By influencing silencing at repetitive elements and regulating gene expression programs, CBX3 is studied in pathways linked to cell cycle control, differentiation, and stress responses. Dysregulated HP1γ-associated chromatin states have been reported across multiple cancer and neurodevelopmental research contexts, supporting its use as a target for mechanistic studies of epigenome-driven phenotypes.
HP1γ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CBX3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CBX3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CBX3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CBX3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.