HoxD9 Double Nickase Plasmid (h): sc-404606-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxD9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxD9 Double Nickase Plasmid (h) and HoxD9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXD9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxD9 Antibody (H-2): sc-137134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxD9 Double Nickase Plasmid (h) | sc-404606-NIC | 20 µg | $410.00 | |||
HoxD9 Double Nickase Plasmid (h2) | sc-404606-NIC-2 | 20 µg | $410.00 |
HOXD9 encodes the homeobox transcription factor HoxD9, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and positional identity during embryonic development. As part of HOX gene regulatory networks, HoxD9 modulates transcriptional programs controlling differentiation, morphogenesis, and cell fate decisions, integrating with chromatin-mediated control and developmental signaling pathways. Dysregulated HOXD9 expression and altered HOX program activity have been associated with changes in lineage specification and proliferation programs observed in multiple cancer contexts and other developmental disorders. These features make HOXD9 a useful target for studying transcriptional circuit architecture, enhancer–promoter logic, and developmental gene misexpression mechanisms in human cells.
HoxD9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXD9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXD9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXD9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXD9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.