HoxB3 Double Nickase Plasmid (h): sc-405460-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxB3 Double Nickase Plasmid (h) and HoxB3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXB3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB3 Double Nickase Plasmid (h) | sc-405460-NIC | 20 µg | $410.00 | |||
HoxB3 Double Nickase Plasmid (h2) | sc-405460-NIC-2 | 20 µg | $410.00 |
HOXB3 encodes the homeobox transcription factor HoxB3, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning and segment identity during embryonic development. In human cells, HoxB3 modulates transcriptional programs linked to lineage specification, differentiation, and morphogen-driven signaling networks that coordinate chromatin state and developmental gene expression. Dysregulated HOXB3 expression has been associated with altered differentiation states and oncogenic transcriptional circuitry in several tumor contexts, making it a useful node for studying developmental reprogramming. Experimental interrogation of HOXB3 supports research into gene regulatory networks, epigenetic control of cell fate, and context-dependent transcription factor function.
HoxB3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.