HoxB2 Lentiviral Activation Particles (h): sc-404533-LAC

HOXB2 encodes the homeobox transcription factor HoxB2, a sequence-specific DNA-binding protein that helps establish anterior–posterior patterning and segment identity during embryonic development. HoxB2 regulates gene expression programs controlling cell fate specification, differentiation, and tissue morphogenesis through transcriptional networks that intersect with developmental signaling pathways such as retinoic acid–responsive programs. Aberrant HOXB2 expression has been reported in multiple tumor contexts and developmental disorders, where altered HOX-regulated transcription can influence proliferation, lineage commitment, and migratory phenotypes. In biomedical research, HOXB2 is studied as a driver of developmental gene regulatory circuitry and as a context-dependent modulator of oncogenic transcriptional states.

HoxB2 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient HOXB2 upregulation across a broader range of human cell types.

HoxB2 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the HOXB2 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous HoxB2 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native HOXB2 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.