HoxA5 Antibody (129C1a) recommended for detection of HoxA5 of human origin by WB and IP

HoxA5 Antibody (129C1a): sc-81289

HoxA5 Antibody (129C1a) is rated 3.0 out of 5 by 1.
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5
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Datasheets

    • HoxA5 Antibody (129C1a) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of HoxA5 of human origin
    • recommended for detection of HoxA5 of human origin by WB and IP

I am using HoxA5 (129C1a): sc-81289 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from WB: strong bands in fusion protein/transfected WB: strong bands in fusion protein/transfected lysates. -SCBT QC
Date published: 2014-11-01
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