HoxA11 Double Nickase Plasmid (h): sc-405264-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA11 Double Nickase Plasmid (h) and HoxA11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA11 Antibody (B-11): sc-393440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA11 Double Nickase Plasmid (h) | sc-405264-NIC | 20 µg | $410.00 | |||
HoxA11 Double Nickase Plasmid (h2) | sc-405264-NIC-2 | 20 µg | $410.00 |
HOXA11 encodes the homeobox transcription factor HoxA11, a DNA-binding regulator that contributes to anterior–posterior patterning and tissue-specific differentiation programs during development. In adult contexts, HOXA11 influences transcriptional networks that coordinate cell fate decisions, extracellular matrix remodeling, and hormone-responsive gene expression, particularly in mesenchymal and reproductive tissues. Dysregulated HOXA11 expression or altered regulatory control has been associated with developmental abnormalities and has been investigated in cancers and reproductive disorders where aberrant transcriptional programs and lineage identity are implicated. As a sequence-specific transcriptional regulator, HoxA11 is commonly studied to map enhancer–promoter interactions and define downstream gene networks that shape tissue architecture and function.
HoxA11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.