Date published: 2026-4-5
Visit Santa Cruz Animal Health

1-800-457-3801

SCBT Portrait Logo
Seach Input
Contact Us
Sign In Account
Quick Order
Cart (0)
Hoechst 33258 (CAS 23491-45-4) - chemical structure image
Molecular structure of Hoechst 33258, CAS Number: 23491-45-4
Hoechst 33258 (CAS 23491-45-4) - chemical structure image
Molecular structure of Hoechst 33258, CAS Number: 23491-45-4
Molecular structure of Hoechst 33258, CAS Number: 23491-45-4

Hoechst 33258 (CAS 23491-45-4)

5.0(1)
Write a reviewAsk a question

See product citations (42)

Alternate Names:
Hoechst Dye 33258; HOE 33258; Bisbenzimidazole
Application:
Hoechst 33258 is a fluorescent stain used for labeling DNA in fluorescent microscopy
CAS Number:
23491-45-4
Molecular Weight:
533.88
Molecular Formula:
C25H24N6O•3HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

Ordering Information
Product Citations
Description
Technical Information
Safety Information
SDS & Certificate of Analysis

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride (Hoechst 33258) is a membrane-permeable, adenine-thymine-specific fluorescent stain, that is useful for staining DNA, chromosomes, and nuclei. This product may act to bind to the minor groove in DNA and can be used to detect mycoplasma contamination in tissue culture, to indicate early onset of apoptosis, and to sort living cells based on DNA content. Hoechst 33258 and the related compound Hoechst 33342 trihydrochloride (sc-200908) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs, and both have been shown to affect the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles interestingly Hoechst 33258 increases eflux while Hoechst 33342 trihydrochloride (sc-200908) decreases it.


Hoechst 33258 (CAS 23491-45-4) References

  1. Base-sequence specificity of Hoechst 33258 and DAPI binding to five (A/T)4 DNA sites with kinetic evidence for more than one high-affinity Hoechst 33258-AATT complex.  |  Breusegem, SY., et al. 2002. J Mol Biol. 315: 1049-61. PMID: 11827475
  2. Hoechst 33258 binds to G-quadruplex in the promoter region of human c-myc.  |  Maiti, S., et al. 2003. Biochem Biophys Res Commun. 310: 505-12. PMID: 14521939
  3. Hoechst 33258 selectively inhibits group I intron self-splicing by affecting RNA folding.  |  Disney, MD., et al. 2004. Chembiochem. 5: 1647-52. PMID: 15532034
  4. Binding of Hoechst 33258 and its derivatives to DNA.  |  Bazhulina, NP., et al. 2009. J Biomol Struct Dyn. 26: 701-18. PMID: 19385699
  5. Interaction of DNA minor groove binder Hoechst 33258 with bovine serum albumin.  |  Ojha, H., et al. 2009. Chem Pharm Bull (Tokyo). 57: 481-6. PMID: 19420779
  6. Binding of Hoechst 33258 to chromatin in situ.  |  Stokke, T. and Steen, HB. 1986. Cytometry. 7: 227-34. PMID: 2423306
  7. Recognition of RNA duplex by a neomycin-Hoechst 33258 conjugate.  |  Willis, B. and Arya, DP. 2014. Bioorg Med Chem. 22: 2327-32. PMID: 24630691
  8. Aggregation features and fluorescence of Hoechst 33258.  |  Busto, N., et al. 2015. J Phys Chem B. 119: 4575-81. PMID: 25759973
  9. UVA irradiation of BrU-substituted DNA in the presence of Hoechst 33258.  |  Saha, A., et al. 2018. Bioorg Med Chem. 26: 37-40. PMID: 29170027
  10. Fluorescent determination of micro-quantities of RNA using Hoechst 33258 and binase.  |  Doynikova, AN. and Vekshin, NL. 2019. Anal Biochem. 576: 5-8. PMID: 30958999
  11. Fluorescence anisotropy studies on the Hoechst 33258-DNA interaction: the solvent effect.  |  Amirbekyan, KY., et al. 2021. J Biomol Struct Dyn. 39: 4902-4906. PMID: 32568619
  12. Study of complexes of Hoechst 33258 with poly(rA)-poly(rU) depending on various ionic strengths in the water-saline solution.  |  Vardevanyan, PO., et al. 2022. J Biomol Struct Dyn. 40: 1182-1188. PMID: 32954957
  13. DNA electrochemical biosensor for detection of Alicyclobacillus acidoterrestris utilizing Hoechst 33258 as indicator.  |  Flauzino, JMR., et al. 2021. Bioelectrochemistry. 140: 107801. PMID: 33789176
  14. Hoechst 33258 staining for detecting mycoplasma contamination in cell cultures: a method for reducing fluorescence photobleaching.  |  Battaglia, M., et al. 1994. Biotech Histochem. 69: 152-6. PMID: 7520758
  15. Bis-benzimidazole dyes, Hoechst 33258 and Hoechst 33342: radioiodination, facile purification and subcellular distribution.  |  Harapanhalli, RS., et al. 1994. Nucl Med Biol. 21: 641-7. PMID: 9234322

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Hoechst 33258, 5 ml

sc-394039
5 ml
$158.00

What is the concentration of this product?

Asked by: Don1
Thank you for your question. Hoechst 33258 (CAS 23491-45-4) is provided as 5 ml and the formulation is 20 mM in water. This information is available on our website under the "Technical Information" tab, next to the "Description" tab, below the Ordering Information on the product page.
Answered by: Technical Support
Date published: 2017-02-15
  • y_2026, m_3, d_26, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_394039, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Li et alLi et al. (Pubmed ID 26426041) used the fluorescent stain, Hoechst 33258, to determine C. lanceolata's ability to induce tumor apoptosis. -SCBT Publication Review
Date published: 2015-01-17
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_394039, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
Hoechst 33258 is rated 5.0 out of 5 by 1.
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_394039, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
1-800-457-3801
Visit Santa Cruz Animal Health|Visit San Juan Ranch
Santa Cruz Animal Health is a leading provider of animal health care products. ©2026 Santa Cruz Biotechnology Inc. All Rights Reserved.
Toll-Free: 1-800-457-3801 - Terms & Conditions | Privacy Policy | Legal
“Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.