hnRNP UL1 CRISPR/Cas9 KO Plasmid (h): sc-405017
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP UL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hnRNP UL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP UL1 Antibody (C-2): sc-393975
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP UL1 CRISPR/Cas9 KO Plasmid (h) | sc-405017 | 20 µg | $397.00 |
Overview
HNRNPUL1 encodes the human heterogeneous nuclear ribonucleoprotein UL1, an RNA- and DNA-associated factor that participates in pre-mRNA processing and the coordination of gene expression with genome maintenance. hnRNP UL1 has been linked to DNA damage response programs, including recruitment and regulation of repair-associated complexes at sites of genotoxic stress, thereby influencing replication stress handling and transcription-coupled repair. Through these roles, HNRNPUL1 impacts cellular proliferation and stress adaptation by modulating RNA metabolism and chromatin-associated repair processes. Altered hnRNP UL1 function or expression has been reported in contexts involving genomic instability and dysregulated RNA processing, supporting its relevance in mechanistic studies of disease-associated pathways.
hnRNP UL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HNRNPUL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HNRNPUL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HNRNPUL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP UL1 protein expression.
This CRISPR knockout system enables efficient generation of HNRNPUL1-deficient cell models for investigation of hnRNP UL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HNRNPUL1 exon(s) critical for hnRNP UL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HNRNPUL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hnRNP UL1 CRISPR/Cas9 KO Plasmid (h) and hnRNP UL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HNRNPUL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hnRNP UL1 HDR Plasmid (h) and hnRNP UL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HNRNPUL1 homology arms to support homology-directed repair at defined HNRNPUL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.