Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP U Double Nickase Plasmid (m): sc-424561-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP U Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP U Double Nickase Plasmid (m) and hnRNP U Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hnrnpu. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP U Antibody (3G6): sc-32315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP U Double Nickase Plasmid (m) | sc-424561-NIC | 20 µg | $410.00 | |||
hnRNP U Double Nickase Plasmid (m2) | sc-424561-NIC-2 | 20 µg | $410.00 |
Mouse Hnrnpu encodes hnRNP U, a ubiquitously expressed nuclear RNA- and DNA-binding protein that scaffolds ribonucleoprotein complexes and contributes to chromatin organization. hnRNP U participates in pre-mRNA processing, alternative splicing, and regulation of gene expression by linking nascent transcripts to nuclear architecture and transcriptional machinery. It has been implicated in pathways governing genome stability, RNA metabolism, and cell-cycle–associated transcriptional programs through interactions with long noncoding RNAs and chromatin-associated factors. Dysregulation of HNRNPU-related RNA processing and nuclear organization is associated with neurodevelopmental phenotypes and altered neuronal excitability, making it relevant for mechanistic studies in nervous system biology and gene regulation.
hnRNP U Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hnrnpu locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hnrnpu. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hnrnpu function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hnrnpu-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.