hnRNP L Double Nickase Plasmid (h): sc-402196-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP L Double Nickase Plasmid (h) and hnRNP L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNRNPL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP L Antibody (4D11): sc-32317
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP L Double Nickase Plasmid (h) | sc-402196-NIC | 20 µg | $410.00 | |||
hnRNP L Double Nickase Plasmid (h2) | sc-402196-NIC-2 | 20 µg | $410.00 |
Human HNRNPL encodes hnRNP L, an RNA-binding protein that recognizes CA-rich elements to coordinate pre-mRNA alternative splicing, transcript stabilization, and nucleocytoplasmic RNA processing. hnRNP L functions within spliceosomal and ribonucleoprotein complexes to shape exon inclusion and coupling of transcription with RNA maturation, influencing programs such as cell-cycle control and stress-responsive gene expression. Through regulation of isoform-specific expression, hnRNP L can modulate signaling outputs including MAPK and apoptosis-related pathways at the post-transcriptional level. Dysregulated hnRNP L activity and splicing alterations have been associated with proliferative phenotypes and aberrant RNA processing patterns observed across diverse human diseases, making it a valuable target for mechanistic studies of splicing-dependent gene regulation.
hnRNP L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNRNPL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNRNPL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNRNPL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNRNPL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.