Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP H Double Nickase Plasmid (m): sc-425559-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP H Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP H Double Nickase Plasmid (m) and hnRNP H Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hnrnph1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP H Double Nickase Plasmid (m) | sc-425559-NIC | 20 µg | $410.00 |
Mouse Hnrnph1 encodes heterogeneous nuclear ribonucleoprotein H (hnRNP H), an RNA-binding protein that recognizes G-rich motifs to regulate pre-mRNA splicing, alternative polyadenylation, and broader RNA processing steps that shape transcript isoform output. hnRNP H functions within spliceosomal and co-transcriptional RNA maturation networks, influencing mRNA stability and translation through its impact on exon inclusion and 3′ end formation. Altered hnRNP H activity can shift isoform balances across neuronal and proliferative programs, making Hnrnph1 a useful locus for studying mechanisms linking RNA processing to cell-state regulation and disease-associated transcriptome remodeling. Research on Hnrnph1 also supports investigation of how splicing factor perturbations propagate through gene regulatory circuits and stress-responsive RNA metabolism.
hnRNP H Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hnrnph1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hnrnph1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hnrnph1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hnrnph1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.