Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP A2/B1 Double Nickase Plasmid (h): sc-400635-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP A2/B1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP A2/B1 Double Nickase Plasmid (h) and hnRNP A2/B1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNRNPA2B1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP A2/B1 Antibody (B-7): sc-374053
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A2/B1 Double Nickase Plasmid (h) | sc-400635-NIC | 20 µg | $410.00 | |||
hnRNP A2/B1 Double Nickase Plasmid (h2) | sc-400635-NIC-2 | 20 µg | $410.00 |
HNRNPA2B1 encodes hnRNP A2/B1, an abundant RNA-binding protein that associates with nascent transcripts to coordinate pre-mRNA splicing, mRNA stabilization, and nucleo-cytoplasmic transport. It also contributes to RNA localization and translation control through interactions with spliceosomal components and other ribonucleoprotein granule factors, linking it to stress granule dynamics and post-transcriptional gene regulation programs. hnRNP A2/B1 functions as an m6A “reader” for select transcripts, influencing RNA fate decisions that shape differentiation and cellular stress responses. Dysregulation of HNRNPA2B1 has been associated with altered RNA processing networks in cancer biology and neurodegeneration-relevant pathways, including aberrant RNP assembly and transcriptome remodeling.
hnRNP A2/B1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNRNPA2B1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNRNPA2B1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNRNPA2B1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNRNPA2B1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.