Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP A1 Double Nickase Plasmid (m): sc-420895-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP A1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP A1 Double Nickase Plasmid (m) and hnRNP A1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hnrnpa1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP A1 Antibody (4B10): sc-32301
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A1 Double Nickase Plasmid (m) | sc-420895-NIC | 20 µg | $410.00 | |||
hnRNP A1 Double Nickase Plasmid (m2) | sc-420895-NIC-2 | 20 µg | $410.00 |
Mouse Hnrnpa1 encodes heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), a ubiquitously expressed RNA-binding protein that couples pre-mRNA splicing, mRNA export, and translation through dynamic interactions with spliceosomal components and ribonucleoprotein granules. hnRNP A1 regulates alternative exon choice and RNA metabolism programs that influence cell cycle progression, stress responses, and maintenance of RNA homeostasis. Its activity is linked to nucleocytoplasmic transport and stress granule assembly, connecting RNA processing to pathways engaged during cellular stress and differentiation. Dysregulation of hnRNP A1-mediated splicing and RNA binding has been associated with neurodegeneration and cancer-relevant transcriptional rewiring, making it a useful node for mechanistic studies of RNA processing defects.
hnRNP A1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hnrnpa1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hnrnpa1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hnrnpa1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hnrnpa1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.