Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP A1 Double Nickase Plasmid (h): sc-400704-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- hnRNP A1 Double Nickase Plasmid (h) and hnRNP A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNRNPA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP A1 Antibody (4B10): sc-32301
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A1 Double Nickase Plasmid (h) | sc-400704-NIC | 20 µg | $410.00 | |||
hnRNP A1 Double Nickase Plasmid (h2) | sc-400704-NIC-2 | 20 µg | $410.00 |
HNRNPA1 encodes heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), a multifunctional RNA-binding protein that associates with pre-mRNAs to regulate alternative splicing, mRNA export, and translation. hnRNP A1 participates in spliceosome assembly and exon definition, and it also contributes to telomere maintenance through interactions with telomeric repeat-containing RNA and telomeric DNA-binding factors. By coordinating RNA processing and RNA metabolism, hnRNP A1 influences stress granule dynamics and broader gene expression programs linked to cell growth and differentiation. Dysregulation of HNRNPA1 has been implicated in neurodegeneration and cancer-relevant RNA processing alterations, making it a useful target for dissecting RNA splicing networks and proteostasis pathways.
hnRNP A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNRNPA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNRNPA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNRNPA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNRNPA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.