Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
hnRNP A/B CRISPR/Cas9 KO Plasmid (m): sc-420896
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- hnRNP A/B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the hnRNP A/B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: hnRNP A/B Antibody (G-12): sc-390957
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A/B CRISPR/Cas9 KO Plasmid (m) | sc-420896 | 20 µg | $397.00 |
Overview
Hnrnpab encodes the mouse heterogeneous nuclear ribonucleoprotein A/B (hnRNP A/B), an RNA-binding protein that associates with nascent transcripts to regulate pre-mRNA splicing, alternative exon selection, mRNA stability, and nucleo-cytoplasmic transport. hnRNP A/B participates in co- and post-transcriptional gene regulation within ribonucleoprotein complexes and helps coordinate transcriptome dynamics during cell proliferation and differentiation. Through its roles in RNA processing and post-transcriptional control, perturbation of hnRNP A/B function can influence pathways linked to stress responses, genome maintenance, and proteostasis. Dysregulated hnRNP family activity has been associated with altered RNA metabolism in cancer and neurodegeneration, making Hnrnpab a useful target for mechanistic studies in disease-relevant models.
hnRNP A/B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hnrnpab gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hnrnpab together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hnrnpab open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish hnRNP A/B protein expression.
This CRISPR knockout system enables efficient generation of Hnrnpab-deficient cell models for investigation of hnRNP A/B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hnrnpab exon(s) critical for hnRNP A/B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hnrnpab genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by hnRNP A/B CRISPR/Cas9 KO Plasmid (m) and hnRNP A/B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hnrnpab locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by hnRNP A/B HDR Plasmid (m) and hnRNP A/B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hnrnpab homology arms to support homology-directed repair at defined Hnrnpab target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.