HLA-DMα Antibody (NB-B33): sc-134356

HLA-DMα Antibody (NB-B33) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HLA-DM alpha protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HLA-DM alpha antibody (NB-B33) is available as a non-conjugated format. HLA-DM alpha protein plays a crucial role in the immune response by facilitating the loading of peptides onto major histocompatibility complex (MHC) class II molecules, which are essential for the activation of CD4+ T helper cells. This process is vital for the adaptive immune system, as HLA-DM alpha enables the presentation of extracellular antigens to T cells, thereby initiating a targeted immune response. HLA-DM alpha protein is primarily located in endosomal and lysosomal compartments, as well as on the surface of B cells, where HLA-DM alpha interacts with MHC class II molecules to promote the exchange of peptides. Proper functioning of HLA-DM alpha protein is critical for maintaining immune tolerance and preventing autoimmune diseases, as evidenced by studies linking specific polymorphisms in HLA-DM genes to susceptibility to conditions such as rheumatoid arthritis. Using HLA-DMα (NB-B33) monoclonal antibody in research can provide valuable insights into the mechanisms of antigen presentation and the role of HLA-DM alpha in immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HLA-DMα Antibody (NB-B33) References:

1. HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus.  |  Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
2. The association of HLA-DM genes with rheumatoid arthritis in Eastern France.  |  Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
3. Functional HLA-DM on the surface of B cells and immature dendritic cells.  |  Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
4. Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta.  |  Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
5. The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism.  |  Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
6. Determination of the HLA-DM interaction site on HLA-DR molecules.  |  Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
7. A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules.  |  Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HLA-DMα Antibody (NB-B33) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HLA-DMα Antibody (NB-B33) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HLA-DMα Antibody (NB-B33) Specifications

Alternate Product Name: human leukocyte antigen (HLA) DM alpha

Product Clone Name: NB-B33

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-262

Epitope Target: HLA-DMα

Epitope Species: human

Product Reactivity/Detection: HLA-DMα

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HLA-DMα Antibody (NB-B33): N/A