Histone H2A.X Antibody (938CT5.1.1): sc-517336

Histone H2A.X Antibody (938CT5.1.1) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets Histone H2A.X, also known as X variant Histone H2A or H2AX. Histone H2A.X is a member of the Histone H2A family involved in the nucleosomal organization of chromatin, differing from other H2A variants by a highly conserved C-terminal motif. Histone H2A.X plays a crucial role in DNA damage response. Upon ionizing radiation or DNA double-stranded breaks, H2A.X is rapidly phosphorylated to form γ-H2A.X, facilitating the recognition and repair of damaged DNA. γ-H2A.X initiates the recruitment of repair factors such as Rad50, Rad51, and BRCA1, and forms nuclear foci during class switch recombination in immunoglobulin genes. Anti-Histone H2A.X antibody (938CT5.1.1) is raised against a synthetic peptide corresponding to amino acids 115-143 in the C-terminal region of human Histone H2A.X and is reactive with human-origin H2A.X in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP) applications. This unconjugated Histone H2A.X (938CT5.1.1) antibody allows for versatile use in various research settings, particularly those studying chromatin organization and DNA repair mechanisms. By targeting a key player in genome stability, Histone H2A.X monoclonal antibody (938CT5.1.1) serves as an essential tool for investigating cellular responses to DNA damage and genomic maintenance processes.

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Histone H2A.X Antibody (938CT5.1.1) References:

1. Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139.  |  Rogakou, EP., et al. 2000. J Biol Chem. 275: 9390-5. PMID: 10734083
2. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.  |  Paull, TT., et al. Curr Biol. 10: 886-95. PMID: 10959836
3. Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress.  |  Ward, IM. and Chen, J. 2001. J Biol Chem. 276: 47759-62. PMID: 11673449
4. AID is required to initiate Nbs1/gamma-H2AX focus formation and mutations at sites of class switching.  |  Petersen, S., et al. 2001. Nature. 414: 660-665. PMID: 11740565
5. The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition.  |  Weyemi, U., et al. 2016. Nat Commun. 7: 10711. PMID: 26876487
6. Phosphorylation of Histone H2A.X in Peripheral Blood Mononuclear Cells May Be a Useful Marker for Monitoring Cardiometabolic Risk in Nondiabetic Individuals.  |  Yoon, SR., et al. 2017. Dis Markers. 2017: 2050194. PMID: 28572698
7. The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage.  |  Piquet, S., et al. 2018. Mol Cell. 72: 888-901.e7. PMID: 30344095
8. Phosphorylation state of the histone variant H2A.X controls human stem and progenitor cell fate decisions.  |  Orlando, L., et al. 2021. Cell Rep. 34: 108818. PMID: 33691101
9. Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis.  |  Jeffery, NN., et al. 2021. Epigenetics Chromatin. 14: 37. PMID: 34330317
10. Spotlight on histone H2A variants: From B to X to Z.  |  Herchenröther, A., et al. 2023. Semin Cell Dev Biol. 135: 3-12. PMID: 35365397
11. Structure of the mouse H2A.X gene and physical linkage to the UPS locus on chromosome 9: assignment of the human H2A.X gene to 11q23 by sequence analysis.  |  Porcher, C. and Grandchamp, B. 1995. Genomics. 25: 312-3. PMID: 7774939
12. Characterization of the human histone H2A.X gene. Comparison of its promoter with other H2A gene promoters.  |  Ivanova, VS., et al. 1994. J Biol Chem. 269: 24189-94. PMID: 7929075

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Histone H2A.X Antibody (938CT5.1.1) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-Histone H2A.X Antibody (938CT5.1.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Histone H2A.X Antibody (938CT5.1.1) Specifications

Alternate Product Name: Histone H2A

Product Clone Name: 938CT5.1.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 115-143

Epitope Target: Histone H2A.X

Epitope Species: human

Product Reactivity/Detection: Histone H2A.X

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for Histone H2A.X Antibody (938CT5.1.1): N/A