Histone H10 Double Nickase Plasmid (h): sc-403716-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone H10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone H10 Double Nickase Plasmid (h) and Histone H10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting H1F0. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone H10 Antibody (34): sc-56695
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone H10 Double Nickase Plasmid (h) | sc-403716-NIC | 20 µg | $410.00 | |||
Histone H10 Double Nickase Plasmid (h2) | sc-403716-NIC-2 | 20 µg | $410.00 |
H1F0 encodes the replication-independent linker histone variant Histone H10, which binds nucleosome entry/exit DNA to stabilize higher-order chromatin structure and tune genome accessibility. Its accumulation is commonly associated with cellular differentiation and reduced proliferative potential, reflecting roles in chromatin compaction, transcriptional regulation, and epigenetic state maintenance. By modulating nucleosome spacing and the physical properties of chromatin, Histone H10 influences processes including DNA replication timing, DNA damage responses, and global gene expression programs. Altered H1F0 expression and chromatin organization have been reported across contexts of tumor biology and lineage specification, supporting its use as a marker and mechanistic node in studies of epigenetic dysregulation.
Histone H10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the H1F0 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within H1F0. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt H1F0 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of H1F0-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.