hippocalcin Antibody (G-8): sc-393125
- hippocalcin Antibody (G-8) is a mouse monoclonal IgG2a κ hippocalcin antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 129-175 mapping near the C-terminus of hippocalcin of human origin
- hippocalcin Antibody (G-8) is recommended for detection of hippocalcin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-hippocalcin Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for hippocalcin Antibody (G-8) for WB applications. This reagent is now offered in a bundle with hippocalcin Antibody (G-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
Hippocalcin Antibody (G-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects hippocalcin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-hippocalcin antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hippocalcin is a neuron-specific calcium-binding protein predominantly located in the plasma membrane of brain and retinal tissues, with particularly high expression in hippocampal pyramidal cells. This localization enables hippocalcin to effectively participate in calcium-dependent signaling pathways, which are essential for regulating neuronal functions. Through plasma membrane binding, hippocalcin interacts with calcium ions, facilitating inhibition of rhodopsin kinase and enhancement of phospholipase D2 expression. Hippocalcin′s ability to modulate these signaling pathways is vital for maintaining neural plasticity and supporting associative memory, particularly in contributing to neuronal survival during aging. Loss of hippocalcin expression has been implicated in age-related declines in post-synaptic functions, highlighting hippocalcin′s importance in preserving cognitive health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hippocalcin Antibody (G-8) References:
- Age-related changes in expression of hippocalcin and NVP2 in rat brain. | Furuta, Y., et al. 1999. Neurochem Res. 24: 651-8. PMID: 10344594
- Role of hippocalcin in Ca2+ -induced activation of phospholipase D. | Hyun, JK., et al. 2000. Mol Cells. 10: 669-77. PMID: 11211872
- Hippocalcin in the olfactory epithelium: a mediator of second messenger signaling. | Mammen, A., et al. 2004. Biochem Biophys Res Commun. 322: 1131-9. PMID: 15336960
- Hippocalcin protects against caspase-12-induced and age-dependent neuronal degeneration. | Korhonen, L., et al. 2005. Mol Cell Neurosci. 28: 85-95. PMID: 15607944
- Hippocalcin-deficient mice display a defect in cAMP response element-binding protein activation associated with impaired spatial and associative memory. | Kobayashi, M., et al. 2005. Neuroscience. 133: 471-84. PMID: 15878804
- Hippocalcin functions as a calcium sensor in hippocampal LTD. | Palmer, CL., et al. 2005. Neuron. 47: 487-94. PMID: 16102532
- Hippocalcin increases phospholipase D2 expression through extracellular signal-regulated kinase activation and lysophosphatidic acid potentiates the hippocalcin-induced phospholipase D2 expression. | Oh, DY., et al. 2006. J Cell Biochem. 97: 1052-65. PMID: 16294323
- Hippocalcin: a new solution to an old puzzle. | Brown, DA., et al. 2007. Neuron. 53: 467-8. PMID: 17296544
- Genomic structure and chromosomal mapping of the human and mouse hippocalcin genes. | Masaki, T., et al. 1998. Gene. 225: 117-24. PMID: 9931466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hippocalcin Antibody (G-8) | sc-393125 | 200 µg/ml | $322.00 | |||
hippocalcin Antibody (G-8): m-IgGκ BP-HRP Bundle | sc-523814 | 200 µg Ab, 40 µg BP | $361.00 | |||
hippocalcin Antibody (G-8) AC | sc-393125 AC | 500 µg/ml, 25% agarose | $424.00 | |||
hippocalcin Antibody (G-8) HRP | sc-393125 HRP | 200 µg/ml | $322.00 | |||
hippocalcin Antibody (G-8) FITC | sc-393125 FITC | 200 µg/ml | $336.00 | |||
hippocalcin Antibody (G-8) PE | sc-393125 PE | 200 µg/ml | $349.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 488 | sc-393125 AF488 | 200 µg/ml | $364.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 546 | sc-393125 AF546 | 200 µg/ml | $364.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 594 | sc-393125 AF594 | 200 µg/ml | $364.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 647 | sc-393125 AF647 | 200 µg/ml | $364.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 680 | sc-393125 AF680 | 200 µg/ml | $364.00 | |||
hippocalcin Antibody (G-8) Alexa Fluor® 790 | sc-393125 AF790 | 200 µg/ml | $364.00 |