HHV-8 K14 Antibody (OX113): sc-53072
- HHV-8 K14 Antibody (OX113) is a mouse monoclonal IgG1 provided at 200 µg/ml
- raised against purified K14 recombinant protein
- HHV-8 K14 Antibody (OX113) is recommended for detection of HHV-8 K14 of viral origin by IF, IHC(P) and FCM
- Anti-HHV-8 K14 Antibody (OX113) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HHV-8 K14 Antibody (OX113). This work is in progress.
HHV-8 K14 Antibody (OX113) is a mouse monoclonal IgG1 antibody that detects HHV-8 K14 protein of viral origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). HHV-8 K14 (OX113) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HHV-8, also known as Kaposi′s sarcoma-associated herpesvirus, is linked to several serious conditions, including multicentric Castleman′s disease and primary effusion lymphoma, a rare form of non-Hodgkin lymphoma that affects body cavities. HHV-8 K14 protein is notably expressed on the surfaces of infected cells exclusively during the lytic cycle, which is crucial for viral replication and spread. HHV-8 K14 interacts with human CD200R, a receptor found on myeloid cells that plays a significant role in modulating immune responses by locally restricting macrophage activation. The structural similarity between HHV-8 K14 and CD200, which shares 40% sequence identity, allows HHV-8 to effectively mimic CD200 activity, thereby inhibiting tumor necrosis factor-alpha production and dampening the immune response. This interaction is vital for viral evasion of the host immune system, highlighting HHV-8 K14′s importance in viral pathogenesis and potential as a target for therapeutic intervention.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HHV-8 K14 Antibody (OX113) References:
- Transcriptional analysis of human herpesvirus-8 open reading frames 71, 72, 73, K14, and 74 in a primary effusion lymphoma cell line. | Talbot, SJ., et al. 1999. Virology. 257: 84-94. PMID: 10208923
- Expression of the open reading frame 74 (G-protein-coupled receptor) gene of Kaposi's sarcoma (KS)-associated herpesvirus: implications for KS pathogenesis. | Kirshner, JR., et al. 1999. J Virol. 73: 6006-14. PMID: 10364352
- Reactivation and role of HHV-8 in Kaposi's sarcoma initiation. | Ensoli, B., et al. 2001. Adv Cancer Res. 81: 161-200. PMID: 11430595
- HHV-8 infection: a model for reactivation and transmission. | Corey, L., et al. 2002. Rev Med Virol. 12: 47-63. PMID: 11787083
- Update on Kaposi's sarcoma and other HHV8 associated diseases. Part 2: pathogenesis, Castleman's disease, and pleural effusion lymphoma. | Hengge, UR., et al. 2002. Lancet Infect Dis. 2: 344-52. PMID: 12144897
- Kaposis sarcoma: the role of HHV-8 and HIV-1 in pathogenesis. | Foreman, KE. 2001. Expert Rev Mol Med. 2001: 1-17. PMID: 14987376
- RBP-J (CSL) is essential for activation of the K14/vGPCR promoter of Kaposi's sarcoma-associated herpesvirus by the lytic switch protein RTA. | Liang, Y. and Ganem, D. 2004. J Virol. 78: 6818-26. PMID: 15194757
- Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor. | Foster-Cuevas, M., et al. 2004. J Virol. 78: 7667-76. PMID: 15220441
- Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 replication and transcription activator regulates viral and cellular genes via interferon-stimulated response elements. | Zhang, J., et al. 2005. J Virol. 79: 5640-52. PMID: 15827179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HHV-8 K14 Antibody (OX113) | sc-53072 | 200 µg/ml | $322.00 | |||
HHV-8 K14 Antibody (OX113) AC | sc-53072 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HHV-8 K14 Antibody (OX113) HRP | sc-53072 HRP | 200 µg/ml | $322.00 | |||
HHV-8 K14 Antibody (OX113) FITC | sc-53072 FITC | 200 µg/ml | $336.00 | |||
HHV-8 K14 Antibody (OX113) PE | sc-53072 PE | 200 µg/ml | $349.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 488 | sc-53072 AF488 | 200 µg/ml | $364.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 546 | sc-53072 AF546 | 200 µg/ml | $364.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 594 | sc-53072 AF594 | 200 µg/ml | $364.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 647 | sc-53072 AF647 | 200 µg/ml | $364.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 680 | sc-53072 AF680 | 200 µg/ml | $364.00 | |||
HHV-8 K14 Antibody (OX113) Alexa Fluor® 790 | sc-53072 AF790 | 200 µg/ml | $364.00 |