HES4 Antibody (D-12): sc-376002
- HES4 Antibody (D-12) is a mouse monoclonal IgG1 κ HES4 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 142-221 mapping near the C-terminus of HES4 of human origin
- HES4 Antibody (D-12) is recommended for detection of HES4 of human origin by WB, IP, IF and ELISA
- Anti-HES4 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-376002 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HES4 Antibody (D-12). This work is in progress.
HES4 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HES4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HES4 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HES4 is a member of the basic helix-loop-helix (bHLH) transcription factor family, which plays a crucial role in gene expression regulation during embryonic development, particularly in the Notch signaling pathway. This pathway is essential for proper segmentation and neural development, influencing cell fate decisions and maintaining the balance between differentiation and proliferation. HES4, along with other HES family members, forms complexes with TLE proteins, which are mammalian homologues of the Drosophila Groucho protein, to repress transcription by binding to specific DNA sequences. HES4 is involved in myogenesis and neurogenesis regulation, making HES4 a significant player in developmental biology. HES4 expression is observed in various adult and embryonic tissues, highlighting HES4′s importance in both developmental processes and potential implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HES4 Antibody (D-12) References:
- The bHLH gene Hes6, an inhibitor of Hes1, promotes neuronal differentiation. | Bae, S., et al. 2000. Development. 127: 2933-43. PMID: 10851137
- Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. | Sasai, Y., et al. 1992. Genes Dev. 6: 2620-34. PMID: 1340473
- Molecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system. | Akazawa, C., et al. 1992. J Biol Chem. 267: 21879-85. PMID: 1400497
- Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements. | Takebayashi, K., et al. 1994. J Biol Chem. 269: 5150-6. PMID: 7906273
- Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split. | Ishibashi, M., et al. 1993. Eur J Biochem. 215: 645-52. PMID: 8354270
- The WRPW motif of the hairy-related basic helix-loop-helix repressor proteins acts as a 4-amino-acid transcription repression and protein-protein interaction domain. | Fisher, AL., et al. 1996. Mol Cell Biol. 16: 2670-7. PMID: 8649374
- Molecular interaction between TLE1 and the carboxyl-terminal domain of HES-1 containing the WRPW motif. | Grbavec, D. and Stifani, S. 1996. Biochem Biophys Res Commun. 223: 701-5. PMID: 8687460
- Expression of the helix-loop-helix factor, Hes3, during embryo development suggests a role in early midbrain-hindbrain patterning. | Lobe, CG. 1997. Mech Dev. 62: 227-37. PMID: 9152013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HES4 Antibody (D-12) | sc-376002 | 200 µg/ml | $322.00 | |||
HES4 Antibody (D-12) X | sc-376002 X | 200 µg/0.1 ml | $322.00 | |||
HES4 Antibody (D-12) AC | sc-376002 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HES4 Antibody (D-12) HRP | sc-376002 HRP | 200 µg/ml | $322.00 | |||
HES4 Antibody (D-12) FITC | sc-376002 FITC | 200 µg/ml | $336.00 | |||
HES4 Antibody (D-12) PE | sc-376002 PE | 200 µg/ml | $349.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 488 | sc-376002 AF488 | 200 µg/ml | $364.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 546 | sc-376002 AF546 | 200 µg/ml | $364.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 594 | sc-376002 AF594 | 200 µg/ml | $364.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 647 | sc-376002 AF647 | 200 µg/ml | $364.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 680 | sc-376002 AF680 | 200 µg/ml | $364.00 | |||
HES4 Antibody (D-12) Alexa Fluor® 790 | sc-376002 AF790 | 200 µg/ml | $364.00 |