HEPES potassium saltA buffering agent used in protein extraction protocols and other biological applications

HEPES potassium salt (CAS 82207-62-3)

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Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt
Application: A buffering agent used in protein extraction protocols and other biological applications
CAS Number: 82207-62-3
Purity: ≥99.5%
Molecular Weight: 276.39
Molecular Formula: C8H17N2O4S•K
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES potassium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt) may be used in the preparation of a HEPES-KOH buffer used in protein extraction protocols and other biological applications. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M. and Heller, W. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (~50 mg/ml).
pH :
useful range: 6.8 - 8.2
Storage :
Store at room temperature
Refractive Index :
n20D 1.54 (Predicted)
pK Values :
pKa: 7.5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
23702134
MDL Number :
MFCD00043438
SMILES :
C1CN(CCN1CCO)CCS(=O)(=O)[O-].[K+]

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Certificate of Analysis

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Could you use this to make a 1M Hepes-KOH, pH 7.5, buffer?

Asked by: SIBS
Thank you for your question. Yes, HEPES potassium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt) may be used in the preparation of a HEPES-KOH buffer used in protein extraction protocols and other biological applications. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.
Answered by: Technical Support
Date published: 2019-01-18
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