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HEPES potassium salt (CAS 82207-62-3)

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Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt

Application: A buffering agent used in protein extraction protocols and other biological applications

CAS Number: 82207-62-3

Purity: ≥99.5%

Molecular Weight: 276.39

Molecular Formula: C₈H₁₇N₂O₄S•K

* Refer to Certificate of Analysis for lot specific data (including water content).

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SDS & Certificate of Analysis

HEPES potassium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt) may be used in the preparation of a HEPES-KOH buffer used in protein extraction protocols and other biological applications. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.

References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M. and Heller, W. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Appearance :

Powder

Physical State :

Solid

Solubility :

Soluble in water (~50 mg/ml).

pH :

useful range: 6.8 - 8.2

Storage :

Store at room temperature

Refractive Index :

n²⁰_D 1.54 (Predicted)

pK Values :

pK_a: 7.5

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

PubChem CID :

23702134

MDL Number :

MFCD00043438

SMILES :

C1CN(CCN1CCO)CCS(=O)(=O)[O-].[K+]

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Certificate of Analysis

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Could you use this to make a 1M Hepes-KOH, pH 7.5, buffer?

Asked by: SIBS

Thank you for your question. Yes, HEPES potassium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt) may be used in the preparation of a HEPES-KOH buffer used in protein extraction protocols and other biological applications. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.

Answered by: Technical Support

Date published: 2019-01-18

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HEPES potassium salt (CAS 82207-62-3)

QUICK LINKS

References

Download SDS (MSDS)

Certificate of Analysis

Could you use this to make a 1M Hepes-KOH, pH 7.5, buffer?

HEPES potassium salt (CAS 82207-62-3)

QUICK LINKS

References

Download SDS (MSDS)

Certificate of Analysis

Could you use this to make a 1M Hepes-KOH, pH 7.5, buffer?

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