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HEPES potassium salt (CAS 82207-62-3)

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Alternate Names:
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid potassium salt
Application:
HEPES potassium salt is a buffering agent used in protein extraction protocols and other biological applications
CAS Number:
82207-62-3
Purity:
≥99.5%
Molecular Weight:
276.39
Molecular Formula:
C8H17KN2O4S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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HEPES potassium salt is a zwitterionic buffering agent used to maintain the pH of biological and chemical solutions in a range of research applications. It is favored for its minimal interference with biochemical reactions and its buffering capacity in the physiological pH range. In cell culture, HEPES potassium salt is used to formulate media and buffer systems that support optimal growth and viability of cells, particularly in situations where CO2 buffering is insufficient or impractical. This buffer is also utilized in protein and nucleic acid research, including electrophoresis and other enzymatic assays, where maintaining a stable pH is essential for accurate results. Moreover, HEPES potassium salt plays a role in the preparation of samples for microscopy, helping to preserve the structural integrity of biological specimens during imaging.


HEPES potassium salt (CAS 82207-62-3) References

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  10. Studies on the interference of selected substances with two modifications of the Lowry protein determination.  |  Himmel, HM. and Heller, W. 1987. J Clin Chem Clin Biochem. 25: 909-13. PMID: 3127532
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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HEPES potassium salt, 25 g

sc-286007
25 g
$67.00

HEPES potassium salt, 100 g

sc-286007A
100 g
$190.00

HEPES potassium salt, 250 g

sc-286007B
250 g
$368.00