HEPES Buffer Solution (1M) (CAS 7365-45-9)

HEPES buffer solution (1M), also known as N-(2-Hydroxyethyl)piperazine-N′-2-ethanesulfonic acid buffer, is a widely used chemical reagent in biological and biochemical research. This buffer is meticulously prepared to maintain a stable pH environment, particularly in cell culture and molecular biology applications where precise pH control is crucial for experimental success. In research, HEPES buffer solution is commonly utilized in cell culture media to maintain a stable pH environment for cell growth and viability. Its non-toxic nature and compatibility with mammalian cell cultures make it a preferred choice for maintaining physiological conditions in vitro. Additionally, HEPES buffer is extensively used in various biochemical assays, enzyme kinetics studies, and molecular biology techniques such as polymerase chain reaction (PCR), gel electrophoresis, and protein biochemistry. The 1M concentration of HEPES buffer solution provides researchers with a convenient stock solution that can be easily diluted to achieve the desired working concentration for specific experimental needs. Its stability and reliability ensure consistent results across experiments, contributing to its widespread use in biological and biochemical laboratories worldwide. Ongoing research in the field continues to explore novel applications and optimizations of HEPES buffer solution, as well as its compatibility with emerging technologies in cell biology, genomics, and proteomics.