Hep C NS5a Antibody (7D4): sc-52417

Hep C NS5a Antibody (7D4) is a mouse monoclonal IgG1 antibody that detects the Hep C NS5a protein of Hep C origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Hep C NS5a antibody (7D4) is available as the non-conjugated format. The Hepatitis C virus, a small, enveloped, single-stranded, positive-sense RNA virus belonging to the Flaviviridae family, primarily targets hepatocytes in the liver for replication. This localization is crucial as viral particles exploit the liver′s cellular machinery for replication, leading to the production of approximately one trillion viral particles daily in infected individuals. Hep C NS5a protein plays a significant role in the viral life cycle, particularly in the regulation of viral RNA replication and assembly. Notably, Hep C NS5a is a phosphoprotein, and phosphorylation state is vital for the efficiency of viral replication, influencing viral ability to persist and evade the host immune response. The high mutation rate of Hep C, attributed to the lack of proofreading by RNA polymerase, further complicates treatment and vaccine development, making the study of proteins like NS5a essential for understanding and combating Hepatitis C infections.

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Hep C NS5a Antibody (7D4) References:

1. The roles of hepatitis C virus proteins in modulation of cellular functions: a novel action mechanism of the HCV core protein on gene regulation by nuclear hormone receptors.  |  Watashi, K. and Shimotohno, K. 2003. Cancer Sci. 94: 937-43. PMID: 14611668
2. Detection and quantitation of HCV core protein in single hepatocytes by means of laser capture microdissection and enzyme-linked immunosorbent assay.  |  Sansonno, D., et al. 2004. J Viral Hepat. 11: 27-32. PMID: 14738555
3. Nucleic acid binding properties and intermediates of HCV core protein multimerization in Pichia pastoris.  |  Acosta-Rivero, N., et al. 2004. Biochem Biophys Res Commun. 323: 926-31. PMID: 15381089
4. Morphological alteration and biological properties of hepatocytes not related to tumorigenesis following transfection with HCV core protein.  |  Gu, J., et al. 2005. J Viral Hepat. 12: 20-6. PMID: 15655044
5. Profiles of HCV core protein and viremia in chronic hepatitis C: possible protective role of core antigen in liver damage.  |  Carabaich, A., et al. 2005. J Med Virol. 76: 55-60. PMID: 15778969
6. HCV core protein augments cyclosporine immunosuppression.  |  Kimball, P., et al. 2005. Transplant Proc. 37: 652-3. PMID: 15848490
7. Thr 446 phosphorylation of PKR by HCV core protein deregulates G2/M phase in HCC cells.  |  Alisi, A., et al. 2005. J Cell Physiol. 205: 25-31. PMID: 15880455
8. Hepatitis C virus (HCV) core protein enhances the immunogenicity of a co-delivered DNA vaccine encoding HCV structural antigens in mice.  |  Alvarez-Lajonchere, L., et al. 2006. Biotechnol Appl Biochem. 44: 9-17. PMID: 16367740
9. Hepatitis C virus NS5A is a direct substrate of casein kinase I-alpha, a cellular kinase identified by inhibitor affinity chromatography using specific NS5A hyperphosphorylation inhibitors.  |  Quintavalle, M., et al. 2007. J Biol Chem. 282: 5536-44. PMID: 17166835
10. Hepatitis C virus NS5A protein enhances gluconeogenesis through upregulation of Akt-/JNK-PEPCK signalling pathways.  |  Kuo, YC., et al. 2014. Liver Int. 34: 1358-68. PMID: 25360475
11. Hepatitis C virus NS5A protein cooperates with phosphatidylinositol 4-kinase IIIα to induce mitochondrial fragmentation.  |  Siu, GK., et al. 2016. Sci Rep. 6: 23464. PMID: 27010100
12. Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase.  |  Yu, CC., et al. 2022. J Virol. 96: e0010722. PMID: 35293767

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hep C NS5a Antibody (7D4) has 5 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hep C NS5a Antibody (7D4) Specifications

Alternate Product Name: Hep C NS5a

Product Clone Name: 7D4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Hep C NS5a

Epitope Species: Hepatitis C virus

Product Reactivity/Detection: an epitope corresponding to amino acids 2190-2300 of NS5a region

Species Reactivity/Detection: Hep C

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Hep C NS5a Antibody (7D4): N/A