Hemoglobin ε Double Nickase Plasmid (h): sc-403392-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hemoglobin ε Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hemoglobin ε Double Nickase Plasmid (h) and Hemoglobin ε Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HBE1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hemoglobin ε Double Nickase Plasmid (h) | sc-403392-NIC | 20 µg | $410.00 | |||
Hemoglobin ε Double Nickase Plasmid (h2) | sc-403392-NIC-2 | 20 µg | $410.00 |
HBE1 encodes hemoglobin ε, an embryonic globin chain that forms functional hemoglobin tetramers with α-like globins to support oxygen transport during early human development. Its expression is tightly regulated within the β-globin locus control region and developmental hemoglobin switching program, linking HBE1 to erythroid differentiation and heme/iron handling pathways. Altered regulation of the globin cluster is relevant to hemoglobinopathies and erythropoietic stress, where developmental globin expression patterns can modulate globin chain balance. As a marker of early erythroid programs, hemoglobin ε is frequently used to study lineage commitment and transcriptional control across the β-globin gene cluster.
Hemoglobin ε Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HBE1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HBE1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HBE1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HBE1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.