Date published: 2026-7-11

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Heme Oxygenase 1/HMOX1 Antibody (D-8): sc-136961

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Datasheets
  • Heme Oxygenase 1/HMOX1 Antibody (D-8) is a mouse monoclonal IgG1 κ, cited in 43 publications, provided at 200 µg/ml
  • raised against amino acids 184-288 of Heme Oxygenase 1 of human origin
  • recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA
  • See Heme Oxygenase 1/HMOX1 (A-3): sc-136960 for Heme Oxygenase 1/HMOX1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Heme Oxygenase 1/HMOX1 Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Heme Oxygenase 1/HMOX1 Antibody (D-8) (see ordering information below).
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Heme Oxygenase 1 Antibody (D-8) is a mouse monoclonal IgG1 antibody that detects Heme Oxygenase 1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Heme Oxygenase 1 (HO-1), also known as HSP 32, plays a crucial role in cellular defense mechanisms by catalyzing the degradation of heme into biliverdin, carbon monoxide, and free iron, thereby contributing to antioxidant protection and cellular homeostasis. The production of carbon monoxide by HO-1 is particularly significant as a signaling molecule in the brain, influencing neurotransmission and vascular regulation. This function is vital for maintaining normal physiological processes and protecting against oxidative stress, which can lead to various pathologies. HO-1 is highly inducible in response to stressors such as heavy metals and oxidative conditions, making HO-1 an important biomarker for studies related to inflammation and cellular stress responses. Anti-Heme Oxygenase 1 antibody (D-8) is an essential tool for researchers investigating the protective roles of HO-1 in human health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Heme Oxygenase 1/HMOX1 Antibody (D-8)

sc-136961
200 µg/ml
$322.00

Heme Oxygenase 1/HMOX1 Antibody (D-8): m-IgG Fc BP-HRP Bundle

sc-537380
200 µg Ab; 10 µg BP
$361.00

Heme Oxygenase 1/HMOX1 Antibody (D-8): m-IgGκ BP-HRP Bundle

sc-534631
200 µg Ab; 40 µg BP
$361.00

Heme Oxygenase 1/HMOX1 Antibody (D-8): m-IgG1 BP-HRP Bundle

sc-545166
200 µg Ab; 20 µg BP
$361.00

When using HMOX-1 antibody for detection with flow cytometry what concentration/dilution of the antibody should be used and what should be the incubation time of the antibody with cells?

Asked by: ElaN
Thank you for your question. We recommend using 1 µg antibody per 1 million cells in 100 µl. Recommended incubation time is 15-30 minutes.
Answered by: Tech Support Europe
Date published: 2025-12-23

I am using Heme Oxygenase 1 (D-8): sc-136961 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_7, d_9, h_10CST
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Rated 5 out of 5 by from Well-rounded antibody for Heme Oxygenase 1This is a wonderful antibody for Western, IHC and IF. Strong and specific, it's a great choice for Heme Oxygenase 1 detection.
Date published: 2017-02-07
Rated 4 out of 5 by from Positive results obtained in western usingPositive results obtained in western using HeLa lysates. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from WB: robust band in human recombinant HemeWB: robust band in human recombinant Heme Oxygenase 1 fusion protein. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Strong bands in western blot analysis ofStrong bands in western blot analysis of Heme Oxygenase 1 expression in human spleen tissue extract and SW-13 whole cell lysate. -SCBT QC
Date published: 2015-01-20
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Heme Oxygenase 1/HMOX1 Antibody (D-8) is rated 4.8 out of 5 by 5.
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