Heme Oxygenase 1 Antibody (D-8) recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA

Heme Oxygenase 1 Antibody (D-8): sc-136961

Heme Oxygenase 1 Antibody (D-8) is rated 4.8 out of 5 by 5.
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Datasheets

    • Heme Oxygenase 1 Antibody (D-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 184-288 of Heme Oxygenase 1 of human origin
    • recommended for detection of Heme Oxygenase 1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Heme Oxygenase 1 (A-3): sc-136960 for Heme Oxygenase 1 antibody conjugates, including AC, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.

I am using Heme Oxygenase 1 (D-8): sc-136961 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Well-rounded antibody for Heme Oxygenase 1 This is a wonderful antibody for Western, IHC and IF. Strong and specific, it's a great choice for Heme Oxygenase 1 detection.
Date published: 2017-02-07
Rated 4 out of 5 by from Positive results obtained in western using Positive results obtained in western using HeLa lysates. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from WB: robust band in human recombinant Heme WB: robust band in human recombinant Heme Oxygenase 1 fusion protein. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Strong bands in western blot analysis of Strong bands in western blot analysis of Heme Oxygenase 1 expression in human spleen tissue extract and SW-13 whole cell lysate. -SCBT QC
Date published: 2015-01-20
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