Hdf1 Antibody (C-10): sc-166798
- Hdf1 Antibody (C-10) is a mouse monoclonal IgG2a κ Hdf1 antibody provided at 200 µg/ml
- raised against amino acids 303-602 mapping at the C-terminus of Hdf1 of Saccharomyces cerevisiae origin
- Hdf1 Antibody (C-10) is recommended for detection of Hdf1 of y origin by WB, IP, IF and ELISA
- Anti-Hdf1 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Hdf1 Antibody (C-10) for WB applications. These reagents are now offered in bundles with Hdf1 Antibody (C-10) (see ordering information below).
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Hdf1 Antibody (C-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Hdf1 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hdf1 (C-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hdf1 protein, also known as Ku-70, is crucial for maintaining telomere length, which is vital for genomic stability and cellular longevity. Hdf1 forms a heterodimer with Ku-80, and together these proteins bind to double-stranded DNA breaks, facilitating DNA repair processes and protecting telomeres from degradation. This protective role is essential in preventing chromosomal end-to-end fusions and ensuring proper cell division. Additionally, Hdf1 interacts with several proteins, including the transcription regulator Rap1, which is involved in telomere position effect silencing and the regulation of adjacent gene expression. The role of Hdf1 in these critical cellular processes underscores its importance in maintaining the integrity of the yeast genome, making anti-Hdf1 antibody (C-10) a valuable tool for researchers studying telomere biology and DNA repair mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hdf1 Antibody (C-10) References:
- TEL1, a gene involved in controlling telomere length in S. cerevisiae, is homologous to the human ataxia telangiectasia gene. | Greenwell, PW., et al. 1995. Cell. 82: 823-9. PMID: 7671310
- The carboxy termini of Sir4 and Rap1 affect Sir3 localization: evidence for a multicomponent complex required for yeast telomeric silencing. | Cockell, M., et al. 1995. J Cell Biol. 129: 909-24. PMID: 7744964
- SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. | Palladino, F., et al. 1993. Cell. 75: 543-55. PMID: 8221893
- RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae. | Kyrion, G., et al. 1993. Genes Dev. 7: 1146-59. PMID: 8319907
- The DNA-binding protein Hdf1p (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae. | Porter, SE., et al. 1996. Nucleic Acids Res. 24: 582-5. PMID: 8604297
- Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae. | Tsukamoto, Y., et al. 1997. Nature. 388: 900-3. PMID: 9278054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hdf1 Antibody (C-10) | sc-166798 | 200 µg/ml | $316.00 | |||
Hdf1 Antibody (C-10): m-IgG Fc BP-HRP Bundle | sc-529053 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hdf1 Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-521750 | 200 µg Ab, 40 µg BP | $354.00 | |||
Hdf1 Antibody (C-10): m-IgG2a BP-HRP Bundle | sc-547246 | 200 µg Ab; 10 µg BP | $354.00 | |||
Hdf1 Antibody (C-10) AC | sc-166798 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Hdf1 Antibody (C-10) HRP | sc-166798 HRP | 200 µg/ml | $316.00 | |||
Hdf1 Antibody (C-10) FITC | sc-166798 FITC | 200 µg/ml | $330.00 | |||
Hdf1 Antibody (C-10) PE | sc-166798 PE | 200 µg/ml | $343.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 488 | sc-166798 AF488 | 200 µg/ml | $357.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 546 | sc-166798 AF546 | 200 µg/ml | $357.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 594 | sc-166798 AF594 | 200 µg/ml | $357.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 647 | sc-166798 AF647 | 200 µg/ml | $357.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 680 | sc-166798 AF680 | 200 µg/ml | $357.00 | |||
Hdf1 Antibody (C-10) Alexa Fluor® 790 | sc-166798 AF790 | 200 µg/ml | $357.00 |