HDAC10 Antibody (F-4) recommended for detection of HDAC10 of human origin by WB, IP, IF and ELISA

HDAC10 Antibody (F-4): sc-376121

HDAC10 Antibody (F-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • HDAC10 Antibody (F-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 61-116 mapping near the N-terminal of HDAC10 of human origin
    • recommended for detection of HDAC10 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with HDAC10 (F-4): sc-376121 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very strong band at correct molecular weight Very strong band at correct molecular weight observed by Western Blot for Jurkat and A-375 cells. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Immunofluorescence staining of methanol Immunofluorescence staining of methanol-fixed HeLa cells - Shows strong staining of nuclear and cytoplasmic localization. -SCBT QC
Date published: 2014-02-21
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