HCF1 Double Nickase Plasmid (h): sc-403097-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HCF1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HCF1 Double Nickase Plasmid (h) and HCF1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HCFC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HCF1 Antibody (H-8): sc-390950
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HCF1 Double Nickase Plasmid (h) | sc-403097-NIC | 20 µg | $410.00 | |||
HCF1 Double Nickase Plasmid (h2) | sc-403097-NIC-2 | 20 µg | $410.00 |
HCFC1 encodes host cell factor 1 (HCF1), a chromatin-associated transcriptional coregulator that coordinates cell-cycle progression and transcriptional programs by bridging sequence-specific transcription factors with epigenetic and coactivator complexes. HCF1 contributes to regulation of E2F-dependent genes, histone modification dynamics, and mitotic progression, supporting proliferative control and cellular homeostasis. Through these interactions, HCF1 influences pathways involved in DNA replication, stress responses, and lineage-specific gene expression. Variants and dysregulation of HCFC1 have been linked to neurodevelopmental phenotypes and altered transcriptional control, making it a relevant target for mechanistic studies in human cells.
HCF1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HCFC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HCFC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HCFC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HCFC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.