hCAP-H Antibody (H-6): sc-101013

hCAP-H Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects hCAP-H protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-hCAP-H antibody (H-6) is available as the non-conjugated form. hCAP-H protein, also known as NCAPH (Non-SMC condensin I complex subunit H), BRRN, BRRN1, or CAPH, is a crucial 741 amino acid component of the condensin complex, which plays a vital role in the organization and segregation of chromosomes during cell division. hCAP-H protein is primarily localized in the cytoplasm and nucleus during interphase and concentrates in the nucleus during mitosis, highlighting its importance in dynamic chromatin remodeling processes. The condensin complex, of which hCAP-H is a regulatory subunit, is essential for converting interphase chromatin into condensed chromosomes, a process critical for accurate chromosome segregation. Furthermore, hCAP-H undergoes post-translational modifications, particularly phosphorylation by Cdc2 (cell division cycle 2), which activates the condensin complex and enables chromosome condensation. This regulatory mechanism underscores hCAP-H′s importance in maintaining genomic stability and proper cell division, making hCAP-H a significant target for research in cell biology and cancer studies.

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hCAP-H Antibody (H-6) References:

1. Chromosome condensation by a human condensin complex in Xenopus egg extracts.  |  Kimura, K., et al. 2001. J Biol Chem. 276: 5417-20. PMID: 11136719
2. Cell cycle-dependent expression and nucleolar localization of hCAP-H.  |  Cabello, OA., et al. 2001. Mol Biol Cell. 12: 3527-37. PMID: 11694586
3. Cnd2 has dual roles in mitotic condensation and interphase.  |  Aono, N., et al. 2002. Nature. 417: 197-202. PMID: 12000964
4. Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair.  |  Heale, JT., et al. 2006. Mol Cell. 21: 837-48. PMID: 16543152
5. Phosphoproteome analysis of the human mitotic spindle.  |  Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
6. A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.  |  Hirano, T. and Mitchison, TJ. 1994. Cell. 79: 449-58. PMID: 7954811
7. Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II.  |  Bhat, MA., et al. 1996. Cell. 87: 1103-14. PMID: 8978614
8. Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2.  |  Cabello, OA., et al. 1997. Genomics. 46: 311-3. PMID: 9417923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hCAP-H Antibody (H-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-hCAP-H Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hCAP-H Antibody (H-6) Specifications

Alternate Product Name: hCAP-H

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 645-741

Epitope Target: hCAP-H

Epitope Species: human

Product Reactivity/Detection: hCAP-H

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for hCAP-H Antibody (H-6): N/A