HAUSP Antibody (H-12): sc-137008

HAUSP Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HAUSP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HAUSP Antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HAUSP, also known as herpesvirus-associated ubiquitin-specific protease (USP7), plays a critical role in regulating the stability of tumor suppressor protein p53 by deubiquitinating p53, which is essential for maintaining cellular homeostasis and preventing tumorigenesis. HAUSP is predominantly localized in the nucleus, where HAUSP associates with nuclear structures known as ND10, which are involved in various cellular processes such as stress response, oncogenesis, and viral infection. HAUSP binding to ND10 is significant because this interaction highlights HAUSP′s role in modulating cellular responses to stress and potential implications in cancer biology. Additionally, HAUSP binds to Vmw110, a regulatory protein from herpesvirus, which can disrupt ND10, further emphasizing HAUSP′s importance in viral pathogenesis and cellular defense mechanisms. HAUSP gene is located on human chromosome band 16p13.2, underscoring HAUSP′s relevance in human health and disease.

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HAUSP Antibody (H-12) References:

1. A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains.  |  Zapata, JM., et al. 2001. J Biol Chem. 276: 24242-52. PMID: 11279055
2. Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus.  |  Muratani, M., et al. 2002. Nat Cell Biol. 4: 106-10. PMID: 11753375
3. Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization.  |  Li, M., et al. 2002. Nature. 416: 648-53. PMID: 11923872
4. Role of deubiquitylase HAUSP in stem cell maintenance.  |  Huang, Z., et al. 2011. Cell Cycle. 10: 1182-3. PMID: 21490432
5. Expression of HAUSP in gliomas correlates with disease progression and survival of patients.  |  Cheng, C., et al. 2013. Oncol Rep. 29: 1730-6. PMID: 23483195
6. Herpesvirus-associated ubiquitin-specific protease (HAUSP) modulates peroxisome proliferator-activated receptor γ (PPARγ) stability through its deubiquitinating activity.  |  Lee, KW., et al. 2013. J Biol Chem. 288: 32886-96. PMID: 24072712
7. Annexin-1 regulated by HAUSP is essential for UV-induced damage response.  |  Park, JJ., et al. 2015. Cell Death Dis. 6: e1654. PMID: 25695607
8. Protein Stability of Pyruvate Kinase Isozyme M2 Is Mediated by HAUSP.  |  Choi, HS., et al. 2020. Cancers (Basel). 12: PMID: 32545446
9. PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6.  |  Kim, JS., et al. 2022. Front Immunol. 13: 862628. PMID: 35572598
10. The disruption of ND10 during herpes simplex virus infection correlates with the Vmw110- and proteasome-dependent loss of several PML isoforms.  |  Everett, RD., et al. 1998. J Virol. 72: 6581-91. PMID: 9658103
11. The ability of herpes simplex virus type 1 immediate-early protein Vmw110 to bind to a ubiquitin-specific protease contributes to its roles in the activation of gene expression and stimulation of virus replication.  |  Everett, RD., et al. 1999. J Virol. 73: 417-26. PMID: 9847347
12. Assignment1 of herpesvirus-associated ubiquitin-specific protease gene HAUSP to human chromosome band 16p13.3 by in situ hybridization.  |  Robinson, PA., et al. 1998. Cytogenet Cell Genet. 83: 100. PMID: 9925944

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAUSP Antibody (H-12) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-HAUSP Antibody (H-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAUSP Antibody (H-12) Specifications

Alternate Product Name: HAUSP

Product Clone Name: H-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 11-210

Epitope Target: HAUSP

Epitope Species: human

Product Reactivity/Detection: HAUSP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for HAUSP Antibody (H-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin