HAUSP Antibody (G-10): sc-376912
- HAUSP Antibody (G-10) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 17-53 near the N-terminus of HAUSP of human origin
- recommended for detection of HAUSP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HAUSP Antibody (G-10) for WB and IHC(P) applications. These reagents are now offered in bundles with HAUSP Antibody (G-10) (see ordering information below).
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HAUSP Antibody (G-10) is a mouse monoclonal IgG1 antibody that detects HAUSP (herpesvirus-associated ubiquitin-specific protease, USP7) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HAUSP plays a critical role in the regulation of p53, a key tumor suppressor protein, by deubiquitinating p53, which is essential for p53 stabilization and function. This interaction is particularly important because p53 is a crucial player in cellular responses to stress, DNA damage, and oncogenic signals, thereby influencing cell cycle regulation and apoptosis. HAUSP can stabilize p53 even in the presence of excess Mdm2, an E3 ubiquitin ligase that targets p53 for degradation, underscoring HAUSP′s significance in cancer biology and therapeutic strategies. HAUSP is predominantly localized in the nucleus, where HAUSP forms a micropunctate pattern and associates with ND10 nuclear structures, which are involved in various cellular processes, including the response to viral infections and oncogenesis. The gene encoding HAUSP is located on human chromosome band 16p13.2, highlighting HAUSP′s evolutionary conservation and relevance across species.
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HAUSP Antibody (G-10) References:
- Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization. | Li, M., et al. 2002. Nature. 416: 648-53. PMID: 11923872
- The finger domain of the human deubiquitinating enzyme HAUSP is a zinc ribbon. | Krishna, SS. and Grishin, NV. 2004. Cell Cycle. 3: 1046-9. PMID: 15254399
- ATM-mediated phosphorylations inhibit Mdmx/Mdm2 stabilization by HAUSP in favor of p53 activation. | Meulmeester, E., et al. 2005. Cell Cycle. 4: 1166-70. PMID: 16082221
- Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway. | Hu, M., et al. 2006. PLoS Biol. 4: e27. PMID: 16402859
- HAUSP as a therapeutic target for hematopoietic tumors (review). | Cheon, KW. and Baek, KH. 2006. Int J Oncol. 28: 1209-15. PMID: 16596237
- A role of HAUSP in tumor suppression in a human colon carcinoma xenograft model. | Becker, K., et al. 2008. Cell Cycle. 7: 1205-13. PMID: 18418047
- USP7/HAUSP promotes the sequence-specific DNA binding activity of p53. | Sarkari, F., et al. 2010. PLoS One. 5: e13040. PMID: 20885946
- Regulation of USP7/HAUSP in response to DNA damage: yet another role for ATM. | Khoronenkova, SV. and Dianov, GL. 2012. Cell Cycle. 11: 2409-10. PMID: 22672935
- Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog Signaling. | Zhou, Z., et al. 2015. Dev Cell. 34: 58-72. PMID: 26120032
- Emerging insights into HAUSP (USP7) in physiology, cancer and other diseases. | Bhattacharya, S., et al. 2018. Signal Transduct Target Ther. 3: 17. PMID: 29967688
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HAUSP Antibody (G-10) | sc-376912 | 200 µg/ml | $316.00 | |||
HAUSP Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-540664 | 200 µg Ab; 10 µg BP | $354.00 | |||
HAUSP Antibody (G-10): m-IgG1 BP-HRP Bundle | sc-542281 | 200 µg Ab; 20 µg BP | $354.00 | |||
HAUSP (G-10) Neutralizing Peptide | sc-376912 P | 100 µg/0.5 ml | $68.00 |