HAO1 Antibody (7A1-8H3B): sc-517552

HAO1 Antibody (7A1-8H3B) is a mouse monoclonal IgG antibody that detects HAO1 protein of mouse, rat, and human origin by western blotting (WB). Anti-HAO1 antibody (7A1-8H3B) is available as a non-conjugated format. HAO1, also known as hydroxyacid oxidase 1 or glycolate oxidase 1, is a 370 amino acid protein predominantly expressed in the liver and pancreas, where HAO1 is localized to peroxisomes. This localization is crucial as peroxisomes are essential organelles involved in various metabolic processes, including the oxidation of fatty acids and the detoxification of hydrogen peroxide. HAO1 plays a significant role in organic acid metabolism through 2-hydroxyacid oxidase activity, which requires a flavin cofactor to catalyze the oxidation of 2-hydroxyacids to 2-ketoacids while simultaneously reducing oxygen to hydrogen peroxide. Notably, HAO1 preferentially oxidizes glycolate, producing glyoxylate, which is vital for the peroxisomal synthesis of glycine. Additionally, HAO1 can convert glyoxylate to oxalate, linking HAO1 to the pathophysiology of hyperoxaluria type 1, a condition characterized by the accumulation of oxalate due to defects in the peroxisomal enzyme AGXT. This accumulation can lead to the formation of calcium oxalate precipitates in the kidneys, posing serious health risks. HAO1′s ability to interact with various substrates, including 2-hydroxy fatty acids and L-α-hydroxy acids, further underscores HAO1′s importance in metabolic pathways.

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HAO1 Antibody (7A1-8H3B) References:

1. Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases.  |  Jones, JM., et al. 2000. J Biol Chem. 275: 12590-7. PMID: 10777549
2. Identification and expression of a cDNA for human glycolate oxidase.  |  Williams, E., et al. 2000. Biochim Biophys Acta. 1493: 246-8. PMID: 10978532
3. Peroxisomal targeting of mammalian hydroxyacid oxidase 1 requires the C-terminal tripeptide SKI.  |  Recalcati, S., et al. 2001. J Cell Sci. 114: 1625-9. PMID: 11309194
4. Oxidative stress-mediated down-regulation of rat hydroxyacid oxidase 1, a liver-specific peroxisomal enzyme.  |  Recalcati, S., et al. 2003. Hepatology. 38: 1159-66. PMID: 14578854
5. Active site and loop 4 movements within human glycolate oxidase: implications for substrate specificity and drug design.  |  Murray, MS., et al. 2008. Biochemistry. 47: 2439-49. PMID: 18215067
6. Generation of hydrogen peroxide in chloroplasts of Arabidopsis overexpressing glycolate oxidase as an inducible system to study oxidative stress.  |  Fahnenstich, H., et al. 2008. Plant Physiol. 148: 719-29. PMID: 18685041

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HAO1 Antibody (7A1-8H3B) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HAO1 Antibody (7A1-8H3B) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HAO1 Antibody (7A1-8H3B) Specifications

Alternate Product Name: HAO1

Product Clone Name: 7A1-8H3B

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: HAO1

Epitope Species: human

Product Reactivity/Detection: HAO1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 5% glycerol

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HAO1 Antibody (7A1-8H3B): N/A