Hantavirus Antibody (A1C5): sc-57755

Hantavirus Antibody (A1C5) is a mouse monoclonal IgG1 antibody that detects the Hantavirus protein by western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Hantavirus antibody (A1C5) is available as the non-conjugated format. Hantaviruses, which belong to the bunyaviridae family, possess a negative-sensed, single-stranded RNA genome composed of three segments: small (S), medium (M), and large (L). The S segment encodes the nucleocapsid protein, while the M segment encodes a polyprotein that is cleaved to produce the envelope glycoproteins G1 and G2. The L segment encodes the L protein, which serves as the viral transcriptase/replicase, playing a crucial role in the transcription of Hantavirus genes. The interaction between the L protein and the nucleocapsid is essential for initiating transcription, highlighting the importance of this protein in the viral life cycle. Furthermore, the G1 and G2 glycoproteins form hetero-oligomers that are transported from the endoplasmic reticulum to the Golgi complex, where they undergo glycosylation, a critical post-translational modification that affects their stability and function. The assembly of Hantavirus virions occurs through the association of nucleocapsids with glycoproteins embedded in the Golgi membranes, underscoring the intricate processes involved in Hantavirus pathogenesis, which can lead to severe diseases such as Hemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus (Cardio-)Pulmonary Syndrome (HPS or HCPS).

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Hantavirus Antibody (A1C5) References:

1. Trimeric hantavirus nucleocapsid protein binds specifically to the viral RNA panhandle.  |  Mir, MA. and Panganiban, AT. 2004. J Virol. 78: 8281-8. PMID: 15254200
2. The hantavirus nucleocapsid protein recognizes specific features of the viral RNA panhandle and is altered in conformation upon RNA binding.  |  Mir, MA. and Panganiban, AT. 2005. J Virol. 79: 1824-35. PMID: 15650206
3. Characterization of truncated hantavirus nucleocapsid proteins and their application for serotyping.  |  Li, G., et al. 2006. J Med Virol. 78: 926-32. PMID: 16721853
4. Oligomerization of hantavirus nucleocapsid protein: analysis of the N-terminal coiled-coil domain.  |  Alminaite, A., et al. 2006. J Virol. 80: 9073-81. PMID: 16940519
5. Nucleotide sequence and coding capacity of the large (L) genomic RNA segment of Seoul 80-39 virus, a member of the hantavirus genus.  |  Antic, D., et al. 1991. Virus Res. 19: 59-65. PMID: 1840713
6. Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae.  |  Avsic-Zupanc, T., et al. 1995. J Gen Virol. 76 (Pt 11): 2801-8. PMID: 7595387
7. Phylogenetic analyses of virus isolates in the genus Hantavirus, family Bunyaviridae.  |  Xiao, SY., et al. 1994. Virology. 198: 205-17. PMID: 8259656
8. Isolation, genetic diversity, and geographic distribution of Bayou virus (Bunyaviridae: hantavirus).  |  Ksiazek, TG., et al. 1997. Am J Trop Med Hyg. 57: 445-8. PMID: 9347961