Date published: 2026-4-26

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HA-Tag Antibody (12CA5): sc-57592

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Datasheets
  • HA-Tag Antibody (12CA5) is a mouse monoclonal IgG2b κ, cited in 242 publications, provided at 50 µg/0.5 ml
  • raised against recombinant influenza virus hemagglutinin
  • recommended for detection of proteins containing the HA tag by WB and IP
  • See HA-Tag (F-7): sc-7392 for HA-Tag antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HA-Tag Antibody (12CA5). This work is in progress.
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HA-Tag Antibody (153) is a mouse monoclonal IgG2a kappa light chain antibody raised against the HA peptide, derived from the influenza hemagglutinin protein. Anti-HA-Tag antibody (153) is widely used to detect HA-tagged fusion proteins in various expression systems, such as bacterial, insect, and mammalian cells. HA-tags consist of an 11 amino acid sequence that aids in purification, localization, and detection of target proteins in experimental settings. The hemagglutinin (HA) protein plays a key role in the influenza virus by facilitating viral entry into host cells through fusion with the host cell membrane, making its structure important for recognition and binding. The HA-tag retains the specific epitope necessary for effective antibody detection, ensuring accurate results in applications like western blotting (WB) and immunohistochemistry. HA-Tag (153) monoclonal antibody binds specifically due to the tag′s accessible and unique sequence. Anti-HA-Tag antibody (153) simplifies detection and purification processes while enabling protein localization studies. HA-Tag (153) antibody is available in both non-conjugated and conjugated forms, making this reagent versatile for various protein research applications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HA-Tag Antibody (12CA5)

sc-57592
50 µg/0.5 ml
$322.00

Is the sc-57592: HA-antibody available conjugated to magnetic beads?

Asked by: Charujack
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for custom orders. Please call our Technical Service Department, or email specialorders@scbt.com.
Answered by: Technical Service 3
Date published: 2020-11-27

Bonjour, Est-il possible d'obtenir un aliquot de l'anticorps: https://www.scbt.com/p/ha-probe-antibody-12ca5? pour tester. Merci!

Asked by: Anonymous
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-02-13

Is sc-57592: HA-probe (12CA5) available conjugated to agarose for IP?

Asked by: cjMara
Thank you for your question. Yes. Please contact one of our Special Orders Coordinators to inquire about the availability of this monoclonal conjugate. Please email specialorders@scbt.com. Or call our Technical Service Department directly at (800) 457-3801 option 2.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 5 out of 5 by from Works great in IHC with cultured myoblastsI bought this antibody two months ago. It works great to detect myoblasts transduced with plasmids expressing HA tag when I do IHC.
Date published: 2023-05-22
Rated 4 out of 5 by from Funziona ma con bande non specificheHo provato un campione di questo prodotto per la detezione di una proteina espressa in protoplasti. La detezione della proteina è stata fatta con un metodo colorimetrico (NBT/BCIP). Però, inoltre alla banda desiderata sono stati visibili anche delle bande non specifiche. Soprattutto con una diluzione dell’anticorpo 1:1000 sono visibili queste bande non specifiche. Con la diluzione più alta (1:1500) si è migliorato un po’ il problema.
Date published: 2018-11-15
Rated 5 out of 5 by from Excellent performanceI asked for it as a free sample, had clean background and a sharp band indicating the HA-tag protein in western blotting
Date published: 2018-10-17
Rated 4 out of 5 by from very good antibody for CO-IP experimentThis Ab pulled down about 50% of HA-tagged protein from nuclear lysate of HEK293T after transient overexpression. A few nonspecific bands were observed (lane 2). Nevertheless, it was very good antibody for CO-IP experiment.
Date published: 2017-01-24
Rated 5 out of 5 by from Excellent detection!Great antibody for detection of HA-tagged proteins!
Date published: 2017-01-10
Rated 4 out of 5 by from Awesome in WB!!!This antibody works very well in western blot detection.
Date published: 2016-12-18
Rated 4 out of 5 by from Works very nicely for IF on human samplesWorks very nicely for IF on human samples. -SCBT QC
Date published: 2014-12-24
Rated 4 out of 5 by from Published in IP with mouse samplesPublished in IP with mouse samples. -SCBT Publication Review
Date published: 2014-11-04
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HA-Tag Antibody (12CA5) is rated 4.4 out of 5 by 10.
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