H3-K9-HMTase Antibody (LD87) recommended for detection of H3-K9-HMTase of human origin by WB and IP

H3-K9-HMTase Antibody (LD87): sc-130475

H3-K9-HMTase Antibody (LD87) is rated 4.5 out of 5 by 2.
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5
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Datasheets

    • Anti-H3-K9-HMTase Antibody (LD87) is a mouse monoclonal IgG2a (kappa light chain) H3-K9-HMTase antibody provided at 50 µg/0.5 ml
    • raised against recombinant H3-K9-HMTase of human origin
    • recommended for detection of H3-K9-HMTase of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of H3-K9-HMTase (LD87): sc-130475.

I am using H3-K9-HMTase (LD87): sc-130475 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Working fairly well with 50 ng of protein Working fairly well with 50 ng of protein lysate loaded.
Date published: 2015-03-31
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